小麦条锈菌的生理小种鉴定

Race Typing of Puccinia striiformis on Wheat.

作者信息

Hovmøller Mogens S, Rodriguez-Algaba Julian, Thach Tine, Sørensen Chris K

机构信息

Department of Agroecology, Aarhus University, Forsogsvej 1, 4200, Slagelse, Denmark.

出版信息

Methods Mol Biol. 2017;1659:29-40. doi: 10.1007/978-1-4939-7249-4_3.

DOI:10.1007/978-1-4939-7249-4_3

PMID:28856638

Abstract

A procedure for virulence phenotyping of isolates of yellow (stripe) rust using spray inoculation of wheat seedlings by spores suspended in an engineered fluid, Novec™ 7100, is presented. Differential sets consisting of near-isogenic Avocet lines, selected lines from the "World" and "European" sets, and additional varieties showing race-specificity facilitate a robust assessment of race, irrespectively of geographical and evolutionary origin of isolates. A simple procedure for purification of samples consisting of multiple races is also presented.

摘要

本文介绍了一种通过将孢子悬浮在工程流体Novec™ 7100中对小麦幼苗进行喷雾接种来对黄色（条）锈病菌株进行毒力表型分析的方法。由近等基因Avocet品系、从“世界”和“欧洲”品系中选出的品系以及其他表现出小种特异性的品种组成的鉴别系，有助于对小种进行可靠评估，而不论菌株的地理和进化起源如何。本文还介绍了一种简单的方法，用于纯化由多个小种组成的样本。