Hamada Moriyuki, Saitou Satomi, Tamura Tomohiko
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3318-3322. doi: 10.1099/ijsem.0.002113. Epub 2017 Aug 31.
A novel Gram-stain-positive actinobacterium, designated HIs16-32T, was isolated from a sand sample collected from a mangrove tidal flat in Japan and its taxonomic position was investigated by a polyphasic approach. The cells of strain HIs16-32T were Gram-stain-positive, rod-shaped, non-motile and non-endospore-forming. Strain HIs16-32T contained glutamic acid, glycine and lysine in the peptidoglycan; however, alanine was absent. Galactose and mannose were detected as cell-wall sugars. The isoprenoid quinones were identified as MK-11, MK-12 and MK-10, and the major fatty acids as anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The DNA G+C content was determined to be 72.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIs16-32T was related to members of the family Microbacteriaceae but did not form a reliable cluster with any known members of the family. The highest 16S rRNA gene sequence similarity values were obtained with species of the genera Herbiconiux(96.1-96.8 %), Plantibacter(96.5-96.7 %) and Schumannella (96.7 %). However, strain HIs16-32T was distinguishable from the phylogenetically related genera in terms of chemotaxonomic characteristics and phylogenetic relationship. Therefore, strain HIs16-32T is concluded to represent a novel genus and species of the family Microbacteriaceae, for which the name Arenivirga flava gen. nov., sp. nov. is proposed. The type strain of A. flava is HIs16-32T (=NBRC 112289T=TBRC 7038T).
从日本红树林潮滩采集的一份沙子样本中分离出一株新型革兰氏阳性放线菌，命名为HIs16 - 32T，并采用多相分类法对其分类地位进行了研究。菌株HIs16 - 32T的细胞革兰氏染色呈阳性，杆状，不运动，不形成芽孢。菌株HIs16 - 32T的肽聚糖中含有谷氨酸、甘氨酸和赖氨酸；然而，不存在丙氨酸。检测到半乳糖和甘露糖为细胞壁糖类。类异戊二烯醌被鉴定为MK - 11、MK - 12和MK - 10，主要脂肪酸为anteiso - C15 : 0、anteiso - C17 : 0和iso - C16 : 0。DNA G + C含量测定为72.2 mol%。基于16S rRNA基因序列比较的系统发育分析表明，菌株HIs16 - 32T与微杆菌科成员相关，但未与该科任何已知成员形成可靠的聚类。与Herbiconiux属（96.1 - 96.8 %）、Plantibacter属（96.5 - 96.7 %）和Schumannella属（96.7 %）的物种获得了最高的16S rRNA基因序列相似性值。然而，菌株HIs16 - 32T在化学分类特征和系统发育关系方面与系统发育相关属不同。因此，得出结论，菌株HIs16 - 32T代表微杆菌科的一个新属和新物种，为此提议命名为Arenivirga flava gen. nov., sp. nov.。A. flava的模式菌株是HIs16 - 32T（=NBRC 112289T=TBRC 7038T）。
