Dyson S, Pinilla M J, Bolas N, Murray R
Centre for Equine Studies, Animal Health Trust, Newmarket, Suffolk, UK.
Centre for Preventative Medicine, Animal Health Trust, Newmarket, Suffolk, UK.
Equine Vet J. 2018 Mar;50(2):159-165. doi: 10.1111/evj.12756. Epub 2017 Sep 13.
There have been no previous studies correlating high-field magnetic resonance imaging (MRI) findings with gross and histological post-mortem examination in horses with hindlimb proximal suspensory desmopathy (PSD).
To determine if adhesions between the suspensory ligament (SL) and adjacent tissues could be detected using high-field MRI and if collagen, adipose tissue and muscle pathology could be differentiated.
Retrospective study.
Nineteen horses with hindlimb PSD were humanely destroyed; 12 limbs of 11 horses with PSD underwent high-field MRI. All lame limbs were examined grossly and all SLs were examined histologically. The MR and histological images were graded blindly. Statistical analysis used a numerical Bayesian approach to simultaneously determine a pathology rating and the contribution of each MRI, gross post-mortem and histological observation to this measure.
Signal intensity (SI) in the collagenous tissue was abnormal in 2/12 limbs. Abnormalities of SI in the adipose tissue and muscle were identified in 6 and 11 limbs, respectively. Adhesions between the SL and adjacent tissues were suspected in eight limbs, but only confirmed in six limbs. Histopathological observations of abnormal muscle tissue were associated most closely with the measure of disease used in the study and were abnormal in every limb. Gross adhesions were also a sensitive indicator, along with changes to nerves which were observed in 10 limbs. Assessments of adipose tissue signal intensity and muscle tissue signal homogeneity in both lobes of the suspensory ligament were the MRI indicators most closely associated with the measure of disease.
Small sample size. Model assumption that all abnormalities reflected degrees of a single disease.
Adhesion formation between the SL and adjacent structures was detected by high-field MRI with reasonable accuracy. Muscle and adipose tissue pathology was identified either alone or in association with collagenous tissue pathology.
此前尚无研究将高场磁共振成像（MRI）结果与后肢近端悬韧带病（PSD）马匹的大体及组织学尸检结果相关联。
确定能否使用高场MRI检测悬韧带（SL）与相邻组织之间的粘连，以及能否区分胶原蛋白、脂肪组织和肌肉的病变。
回顾性研究。
对19匹患有后肢PSD的马匹实施安乐死；11匹患有PSD的马匹的12条肢体接受了高场MRI检查。对所有跛行肢体进行大体检查，并对所有SL进行组织学检查。对MR图像和组织学图像进行盲法分级。统计分析采用数值贝叶斯方法，以同时确定病理分级以及每个MRI、大体尸检和组织学观察结果对该指标的贡献。
12条肢体中有2条肢体的胶原组织信号强度（SI）异常。脂肪组织和肌肉的SI异常分别在6条和11条肢体中被发现。8条肢体疑似SL与相邻组织之间存在粘连，但仅在6条肢体中得到证实。异常肌肉组织的组织病理学观察结果与研究中使用的疾病指标关联最为密切，且在每条肢体中均有异常。大体粘连以及在10条肢体中观察到的神经变化也是敏感指标。对悬韧带两叶脂肪组织信号强度和肌肉组织信号均匀性的评估是与疾病指标关联最为密切的MRI指标。
样本量小。模型假设所有异常均反映单一疾病的程度。
高场MRI能以合理的准确性检测出SL与相邻结构之间粘连的形成。肌肉和脂肪组织病变可单独或与胶原组织病变一起被识别。
