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绵羊嗅球中儿茶酚胺和5-羟色胺能神经元存在的免疫细胞化学证明。

Immunocytochemical demonstration of the presence of catecholamine and serotonin neurons in the sheep olfactory bulb.

作者信息

Tillet Y, Thibault J, Dubois M P

出版信息

Neuroscience. 1987 Mar;20(3):1011-22. doi: 10.1016/0306-4522(87)90259-4.

DOI:10.1016/0306-4522(87)90259-4
PMID:2885778
Abstract

The catecholamine and serotonin innervation of the sheep olfactory bulb was studied using immunocytochemistry. Specific antisera raised against tyrosine hydroxylase, dopamine beta-hydroxylase, phenylethanolamine N-methyl transferase and serotonin were used. Tyrosine hydroxylase-positive cell bodies were present in all cell layers except in the anterior olfactory nucleus, the greatest number being found in the glomerular layer. Neither dopamine beta-hydroxylase-positive nor serotonin-positive cell bodies were observed. Dopamine beta-hydroxylase-positive fibers were widely distributed in the granule cell layer but less widely in other layers. The glomerular layer contained the greatest distribution of serotonergic positive fibers, but such fibers were also visualized in other cell layers. No phenylethanolamine N-methyl transferase-positive structures were found in this investigation.

摘要

利用免疫细胞化学方法研究了绵羊嗅球的儿茶酚胺和5-羟色胺神经支配。使用了针对酪氨酸羟化酶、多巴胺β-羟化酶、苯乙醇胺N-甲基转移酶和5-羟色胺的特异性抗血清。除前嗅核外,所有细胞层均存在酪氨酸羟化酶阳性细胞体,其中在肾小球层发现的数量最多。未观察到多巴胺β-羟化酶阳性或5-羟色胺阳性细胞体。多巴胺β-羟化酶阳性纤维广泛分布于颗粒细胞层,但在其他层分布较少。肾小球层含有最广泛分布的5-羟色胺能阳性纤维,但在其他细胞层也可见到此类纤维。在本研究中未发现苯乙醇胺N-甲基转移酶阳性结构。

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引用本文的文献

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Presence of dopamine-immunoreactive cell bodies in the catecholaminergic group A15 of the sheep brain.绵羊脑儿茶酚胺能A15组中多巴胺免疫反应性细胞体的存在。
Histochemistry. 1990;93(3):327-33. doi: 10.1007/BF00266396.