Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210093, P. R. China
Collaborative Innovation Center of Chemistry for Life Sciences, College of Engineering and Applied Sciences, Nanjing National Laboratory of Microstructures, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, P. R. China
ACS Appl Mater Interfaces. 2017 Sep 20;9(37):31509-31518. doi: 10.1021/acsami.7b09522. Epub 2017 Sep 11.
The development of agents for noninvasive photothermal/photodynamic therapies (PTT/PDT) against cancer remains challenging because most PTT agents cause side effects on normal tissues due to their high cytotoxicity and slow metabolism rate. We successfully synthesized an organic compound (C3), encapsulated in PEG-PCL with indocyanine green (ICG), to form hybrid nanoparticles (PEG-PCL-C3-ICG NPs) for use as a new PPT/PDT agent to treat cancer with a single irradiation. Compared with conventional PPT agents, such as Au nanorods, C3 showed better photothermal conversion stability, lower cytotoxicity and a faster metabolic rate, ensuring promising PTT efficacy in eliminating tumors during in vivo application, while ICG was used as a PDT agent. With 808 nm laser irradiation at tumor sites, the PEG-PCL-C3-ICG NPs were able to simultaneously produce hyperthermia through C3 and produce reactive oxygen species as well as a fluorescence-guided effect through ICG to kill oral squamous cell carcinoma (OSCC) cells. The combination of these hypotoxic and metabolic hybrid nanoparticles with radiation therapy has potential for the future treatment of OSCC.
用于癌症的非侵入性光热/光动力疗法（PTT/PDT）的试剂的开发仍然具有挑战性，因为大多数 PTT 试剂由于其高细胞毒性和缓慢的代谢率而对正常组织造成副作用。我们成功地合成了一种有机化合物（C3），将其封装在聚乙二醇-聚己内酯中，并与吲哚菁绿（ICG）结合，形成用于治疗癌症的新型 PTT/PDT 试剂，可通过单次照射使用。与传统的 PTT 试剂（如金纳米棒）相比，C3 表现出更好的光热转换稳定性、更低的细胞毒性和更快的代谢率，确保在体内应用中消除肿瘤的 PTT 疗效有很大的潜力，而 ICG 则用作 PDT 试剂。在肿瘤部位用 808nm 激光照射时，PEG-PCL-C3-ICG NPs 能够通过 C3 产生高热，并通过 ICG 产生活性氧以及荧光引导作用来杀死口腔鳞状细胞癌（OSCC）细胞。这些低毒性和代谢混合纳米粒子与放射治疗相结合，有望用于未来 OSCC 的治疗。
