Bicknese Alma R, Goodwin Holly S, Quinn Cheryl O, Henderson Verneake C D, Chien Shin-Nan, Wall Donna A
Departments of Neurology, Cardinal Glennon Children's Hospital, St. Louis, MO 63110.
Departments of Pediatrics of Saint Louis University, Cardinal Glennon Children's Hospital, St. Louis, MO 63110.
Cell Transplant. 2002 Apr;11(3):261-264. doi: 10.3727/096020197390022.
Rare cells are present in human umbilical cord blood that do not express the hematopoietic marker CD45 and in culture do not produce cells of hematopoietic lineage. These umbilical cord multipotent stem cells (UC-MC) behave as multilineage progenitor cells (stem cells) and can be expanded in tissue culture. Exposure to basic fibroblast growth factor (bFGF) and human epidermal growth factor (hEGF) for a minimum of 7 days in culture induces expression of neural and glial markers. Western immunoblots demonstrate expression of both β-tubulin III and glial fibrillary acidic protein (GFAP). Immunocytochemistry of the cells showed intense labeling to both compounds on the intracellular cytoskeleton. The oligodendrocyte cell surface marker galactocerebroside (Gal-C) was present on most cells. Many cells show dual labeling, expressing both neuronal and glial markers.
人类脐带血中存在不表达造血标志物CD45的稀有细胞，且在培养过程中不会产生造血谱系细胞。这些脐带多能干细胞（UC-MC）表现为多谱系祖细胞（干细胞），并可在组织培养中扩增。在培养过程中至少7天暴露于碱性成纤维细胞生长因子（bFGF）和人表皮生长因子（hEGF）可诱导神经和神经胶质标志物的表达。蛋白质免疫印迹显示β-微管蛋白III和胶质纤维酸性蛋白（GFAP）均有表达。细胞免疫细胞化学显示细胞内细胞骨架上这两种化合物均有强烈标记。大多数细胞存在少突胶质细胞表面标志物半乳糖脑苷脂（Gal-C）。许多细胞呈现双重标记，同时表达神经元和神经胶质标志物。
