Research Unit of Pharmacology and Toxicology of Xenobiotics (UR12 ES13) and Laboratory of Pharmacology, Faculty of Medicine, University of Sfax, Tunisia.
Research Unit of Pharmacology and Toxicology of Xenobiotics (UR12 ES13) and Laboratory of Pharmacology, Faculty of Medicine, University of Sfax, Tunisia.
Biomed Pharmacother. 2017 Nov;95:308-316. doi: 10.1016/j.biopha.2017.08.039. Epub 2017 Sep 12.
Imipenem is a carbapenem antibiotic largely used to treat infection diseases. The present study was designed to investigate the effects of imipenem/cilastatin (IMP) on oxidative stress, antioxidant levels, testicular structure and sperm parameters in rats. Adult Wistar rats (84days old; N=8/group) were treated intraperitoneally with physiological serum containing 0mg/kg, 30mg/kg, 50mg/kg and 80mg/kg of IMP for one week. The results revealed that exposure to IMP especially at high doses, significantly decreased sexual organs weights (testis, epididymis, seminal vesicle and prostate), sperm characteristics (motility, viability and count) and plasma testosterone level while increased sperm abnormality. In addition, the testicular tissue level of lipid peroxidation (LPO) was significantly increased while the level of activities of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GPx) decreased compared to the control group. Severe testicular lesions were recorded in the seminiferous tubules as well as a significant impairment in sperm characteristics. In conclusion, IMP induced an oxidative stress-status and histopathological changes in the testis and altered spermatogenesis in particular at both 50 and 80mg/kg dose-levels (p<0.001).
亚胺培南是一种碳青霉烯类抗生素，主要用于治疗感染性疾病。本研究旨在探讨亚胺培南/西司他丁（IMP）对大鼠氧化应激、抗氧化水平、睾丸结构和精子参数的影响。成年 Wistar 大鼠（84 天龄；每组 8 只）经腹腔注射含有 0mg/kg、30mg/kg、50mg/kg 和 80mg/kgIMP 的生理盐水，每周处理一次。结果表明，暴露于 IMP 特别是高剂量时，显著降低了生殖器官重量（睾丸、附睾、精囊和前列腺）、精子特性（活力、活力和计数）和血浆睾酮水平，同时增加了精子畸形率。此外，与对照组相比，睾丸组织中脂质过氧化（LPO）水平显著升高，而超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性降低。在曲细精管中记录到严重的睾丸损伤，以及精子特性的显著损害。总之，IMP 诱导了氧化应激状态和睾丸的组织病理学变化，并在 50 和 80mg/kg 剂量水平下改变了精子发生（p<0.001）。
