Department of Cardiovascular and Renal Research, University of Southern Denmark, J.B. Winslowvej 21 3, DK-5000 Odense, Denmark.
Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Sdr. Boulevard 29, DK-5000 Odense, Denmark.
Cardiovasc Res. 2017 Sep 1;113(11):1282-1293. doi: 10.1093/cvr/cvx125.
Cardiovascular diseases remain the leading cause of death worldwide and current treatment strategies have limited effect of disease progression. It would be desirable to have better models to study developmental and pathological processes and model vascular diseases in laboratory settings. To this end, human induced pluripotent stem cells (hiPSCs) have generated great enthusiasm, and have been a driving force for development of novel strategies in drug discovery and regenerative cell-therapy for the last decade. Hence, investigating the mechanisms underlying the differentiation of hiPSCs into specialized cell types such as cardiomyocytes, endothelial cells, and vascular smooth muscle cells (VSMCs) may lead to a better understanding of developmental cardiovascular processes and potentiate progress of safe autologous regenerative therapies in pathological conditions. In this review, we summarize the latest trends on differentiation protocols of hiPSC-derived VSMCs and their potential application in vascular research and regenerative therapy.
心血管疾病仍然是全球范围内的主要死亡原因，目前的治疗策略对疾病进展的影响有限。如果有更好的模型来研究发育和病理过程，并在实验室环境中模拟血管疾病，那就太好了。为此，人类诱导多能干细胞（hiPSC）引起了极大的兴趣，并且在过去十年中一直是药物发现和再生细胞治疗新策略发展的推动力。因此，研究 hiPSC 分化为专门细胞类型（如心肌细胞、内皮细胞和血管平滑肌细胞（VSMC））的机制可能有助于更好地理解发育中的心血管过程，并促进在病理条件下进行安全的自体再生治疗的进展。在这篇综述中，我们总结了 hiPSC 衍生的 VSMC 分化方案的最新趋势及其在血管研究和再生治疗中的潜在应用。
