Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas - UNICAMP, 13083-864, Campinas, São Paulo, Brazil.
Laboratory of Genetics, Butantan Institute, 05503-900, São Paulo, Brazil.
Pathog Dis. 2017 Aug 31;75(6). doi: 10.1093/femspd/ftx074.
Salmonella enterica Enteritidis forms biofilms and survives in agricultural environments, infecting poultry and eggs. Bacteria in biofilms are difficult to eradicate compared to planktonic cells, causing serious problems in industry and public health. In this study, we evaluated the role of ihfA and ihfB in biofilm formation by S. enterica Enteritidis by employing different microbiology techniques. Our data indicate that ihf mutant strains are impaired in biofilm formation, showing a reduction in matrix formation and a decrease in viability and metabolic activity. Phenotypic analysis also showed that deletion of ihf causes a deficiency in curli fimbriae expression, cellulose production and pellicle formation. These results show that integration host factor has an important regulatory role in biofilm formation by S. enterica Enteritidis.
肠炎沙门氏菌肠炎亚种形成生物膜并在农业环境中存活，感染家禽和鸡蛋。与浮游细胞相比，生物膜中的细菌更难消除，这给工业和公共卫生带来了严重问题。在这项研究中，我们使用不同的微生物学技术评估了 ihfA 和 ihfB 在肠炎沙门氏菌生物膜形成中的作用。我们的数据表明，ihf 突变菌株在生物膜形成中受损，基质形成减少，活力和代谢活性降低。表型分析还表明，ihf 的缺失导致卷曲菌毛表达、纤维素产生和菌膜形成缺陷。这些结果表明整合宿主因子在肠炎沙门氏菌生物膜形成中具有重要的调节作用。
