Kim Hyeong Seop, Lee Na Kyung, Yoo Dongkyeom, Lee Jeongmin, Choi Soo Jin, Oh Wonil, Chang Jong Wook, Na Duk L
Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, 06351, Republic of Korea; Department of Neurology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 06351, Republic of Korea; Neuroscience Center, Samsung Medical Center, Seoul, 06351, Republic of Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, 06351, Republic of Korea.
Center for Molecular & Cellular Imaging, Samsung Biomedical Research Institute, Seoul, 06351, Republic of Korea.
Biochem Biophys Res Commun. 2017 Nov 4;493(1):751-757. doi: 10.1016/j.bbrc.2017.08.115. Epub 2017 Aug 30.
Due to their widely known therapeutic benefits, mesenchymal stem cells have been proposed as a novel treatment option for a wide range of diseases including Alzheimer's disease. To maximize these benefits, critical factors such as delivery route, cell viability, and cell migration must be accounted for. Out of the various delivery routes to the brain, the intracerebroventricular (ICV) route stands out due to the widespread distribution that can occur via cerebrospinal fluid flow. The major objective of this present study was to observe how altering cell concentration influences the migration and viability of human umbilical cord blood derived-mesenchymal stem cells (hUCB-MSCs), delivered via ICV injection, in the brains of wild-type (WT) mice. C3H/C57 WT mice were divided into three groups and were injected with 1 × 10 hUCB-MSCs suspended in varying volumes: high (3 μl), middle (5 μl), and low (7 μl) concentrations, respectively. Lowering the concentration increased the migratory capabilities and elevated the viability of hUCB-MSCs. These results suggest that cell concentration can affect the physiological state of hUCB-MSCs, and thus the extent of therapeutic efficacy that can be achieved.
由于间充质干细胞具有广为人知的治疗益处，已被提议作为包括阿尔茨海默病在内的多种疾病的一种新型治疗选择。为了最大化这些益处，必须考虑诸如给药途径、细胞活力和细胞迁移等关键因素。在通往大脑的各种给药途径中，脑室内（ICV）途径因其可通过脑脊液流动实现广泛分布而脱颖而出。本研究的主要目的是观察改变细胞浓度如何影响通过ICV注射递送的人脐带血来源间充质干细胞（hUCB - MSCs）在野生型（WT）小鼠大脑中的迁移和活力。将C3H/C57 WT小鼠分为三组，分别注射悬浮在不同体积中的1×10 hUCB - MSCs：高浓度（3μl）、中浓度（5μl）和低浓度（7μl）。降低浓度可提高hUCB - MSCs的迁移能力并提高其活力。这些结果表明细胞浓度可影响hUCB - MSCs的生理状态，进而影响可实现的治疗效果程度。
