Center for Theragnosis, Korea Institute of Science and Technology, Seoul 02792, Korea.
KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Korea.
Int J Mol Sci. 2021 Jan 15;22(2):845. doi: 10.3390/ijms22020845.
Mesenchymal stem cells (MSCs) have the potential to be a viable therapy against various diseases due to their paracrine effects, such as secretion of immunomodulatory, trophic and protective factors. These cells are known to be distributed within various organs and tissues. Although they possess the same characteristics, MSCs from different sources are believed to have different secretion potentials and patterns, which may influence their therapeutic effects in disease environments. We characterized the protein secretome of adipose (AD), bone marrow (BM), placenta (PL), and Wharton's jelly (WJ)-derived human MSCs by using conditioned media and analyzing the secretome by mass spectrometry and follow-up bioinformatics. Each MSC secretome profile had distinct characteristics depending on the source. However, the functional analyses of the secretome from different sources showed that they share similar characteristics, such as cell migration and negative regulation of programmed cell death, even though differences in the composition of the secretome exist. This study shows that the secretome of fetal-derived MSCs, such as PL and WJ, had a more diverse composition than that of AD and BM-derived MSCs, and it was assumed that their therapeutic potential was greater because of these properties.
间充质干细胞 (MSCs) 由于其旁分泌作用，如分泌免疫调节、营养和保护因子，具有成为各种疾病可行治疗方法的潜力。这些细胞分布在各种器官和组织中。尽管它们具有相同的特征，但来自不同来源的 MSCs 被认为具有不同的分泌潜力和模式，这可能会影响它们在疾病环境中的治疗效果。我们通过使用条件培养基来表征脂肪（AD）、骨髓（BM）、胎盘（PL）和 Wharton 胶（WJ）来源的人 MSCs 的蛋白质分泌组，并通过质谱分析和后续的生物信息学分析来分析分泌组。每个 MSC 分泌组图谱都根据来源具有独特的特征。然而，对不同来源的分泌组的功能分析表明，它们具有相似的特征，如细胞迁移和程序性细胞死亡的负调控，尽管分泌组的组成存在差异。这项研究表明，来自胎儿的 MSCs（如 PL 和 WJ）的分泌组比来自 AD 和 BM 的 MSCs 的分泌组具有更多样的组成，并且由于这些特性，它们的治疗潜力更大。
