植物细胞中胞外分泌复合体阳性细胞器（EXPO）介导的非常规蛋白质分泌（UPS）分析

Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion (UPS) in Plant Cells.

作者信息

Ding Yu, Wang Juan

机构信息

Department of Food Science & Engineering, College of Science and Engineering, Jinan University, Guangzhou, 510632, China.

College of Food Science, South China Agricultural University, Guangzhou, 510642, China.

出版信息

Methods Mol Biol. 2017;1662:231-241. doi: 10.1007/978-1-4939-7262-3_21.

DOI:10.1007/978-1-4939-7262-3_21

PMID:28861833

Abstract

Unconventional protein secretion (UPS) together with conventional protein secretion (CPS) is responsible for protein secretion in plants. We have previously identified a novel UPS pathway in plants, which is mediated by exocyst-positive organelle-EXPO. Here, we describe detailed protocols to study UPS in plants by using Arabidopsis protoplasts or transgenic suspension cells, expressing the EXPO marker Exo70E2-XFP, as materials. Via drug and osmotic treatment plus secretion assay, we illustrate several major methods to analyze EXPO-mediated UPS in plant cells, which also supplys mining tools for similar study.

摘要

非常规蛋白质分泌（UPS）与常规蛋白质分泌（CPS）共同负责植物中的蛋白质分泌。我们之前在植物中鉴定出了一条新的UPS途径，该途径由外泌体阳性细胞器EXPO介导。在这里，我们描述了详细的实验方案，以拟南芥原生质体或表达EXPO标记Exo70E2-XFP的转基因悬浮细胞为材料，研究植物中的UPS。通过药物和渗透处理以及分泌测定，我们阐述了几种分析植物细胞中EXPO介导的UPS的主要方法，这些方法也为类似研究提供了挖掘工具。