豌豆（Pisum sativum, L）幼苗中游离N-聚糖的出现情况。

Occurrence of Free N-Glycans in Pea (Pisum sativum, L) Seedlings.

作者信息

Kimura Yoshinobu, Takagi Shigeaki, Shiraishi Tomonori

机构信息

a Department of Bioresources Chemistry , Tsushima-Naka, Okayama 700 , Japan.

b Department of Biological Function and Genetic Resources Science, Faculty of Agriculture , Okayama University , Tsushima-Naka, Okayama 700 , Japan.

出版信息

Biosci Biotechnol Biochem. 1997 Jan;61(5):924-926. doi: 10.1271/bbb.61.924.

DOI:10.1271/bbb.61.924

PMID:28862564

Abstract

Free N-glycans have been found in pea seedlings. These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con Α-Sepharose affinity chromatography, and size fractionation HPLC. These structures of pyridylaminated free N-glycans were analyzed by exomannosidase digestions and ion- spray tandem mass spectrometry. The structural analyses showed that the several oligomannose-type sugar chains having one GlcNAc residue at the reducing-end side occur in the seedlings, suggesting the endo-β-N-acetylgIucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228-232 (1996)] should be involved in the release of oligomannose-type N-glycans from the storage glycoproteins [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 1841-1850 (1996)] during the germination of pea seeds.

摘要

在豌豆幼苗中发现了游离N-聚糖。这些游离N-聚糖与2-氨基吡啶偶联，并通过凝胶过滤、伴刀豆球蛋白A-琼脂糖亲和色谱和尺寸分级高效液相色谱进行纯化。通过外切甘露糖苷酶消化和离子喷雾串联质谱分析了吡啶胺化游离N-聚糖的这些结构。结构分析表明，在还原端一侧具有一个GlcNAc残基的几种低聚甘露糖型糖链存在于幼苗中，这表明内切β-N-乙酰葡糖胺酶PS [Y. Kimura等人，生物科学、生物技术、生物化学，60，228 - 232 (1996)] 应该参与了豌豆种子萌发过程中低聚甘露糖型N-聚糖从储存糖蛋白 [Y. Kimura等人，生物科学、生物技术、生物化学，60，1841 - 1850 (1996)] 中的释放。