Cheng Kaiying, Xu Guangzhi, Xu Hong, Zhao Ye, Hua Yuejin
Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
Agriculture and Food Science School, Zhejiang Agriculture and Forestry University, Zhejiang, Lin'an 311300, China.
Mol Microbiol. 2017 Nov;106(4):518-529. doi: 10.1111/mmi.13828. Epub 2017 Oct 10.
RecF, together with the recombination mediators RecO and RecR, is required in the RecFOR homologous recombination repair pathway in bacteria. In this study, a recF-dr1088 operon, which is highly conserved in the Deinococcus-Thermus phylum, was identified in Deinococcus radiodurans. Interaction between DRRecF and DR1088 was confirmed by yeast two-hybrid and pull-down assays. DR1088 exhibited some RecO-like biochemical properties including single/double-stranded DNA binding activity, ssDNA binding protein (SSB) replacement ability and ssDNA (with or without SSB) annealing activity. However, unlike other recombination proteins, dr1088 is essential for cell viability. These results indicate that DR1088 might play a role in DNA replication and DNA repair processes.
RecF与重组介质RecO和RecR一起，是细菌RecFOR同源重组修复途径所必需的。在本研究中，在耐辐射球菌中鉴定出了recF-dr1088操纵子，其在嗜热栖热菌门中高度保守。通过酵母双杂交和下拉试验证实了DRRecF与DR1088之间的相互作用。DR1088表现出一些类似RecO的生化特性，包括单链/双链DNA结合活性、单链DNA结合蛋白（SSB）替代能力和单链DNA（有或无SSB）退火活性。然而，与其他重组蛋白不同，dr1088对细胞活力至关重要。这些结果表明，DR1088可能在DNA复制和DNA修复过程中发挥作用。
