Li Xiaoxia, Wu Miaoqin, Zhang Luyi, Liu Hui, Zhang Lan, He Jinjing
Department of Ophthalmology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China.
Department of Ophthalmology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China.
Exp Eye Res. 2017 Dec;165:1-6. doi: 10.1016/j.exer.2017.08.019. Epub 2017 Aug 31.
In this study, we evaluated the effect of oral administration of riboflavin combined with whole-body ultraviolet A (UVA) irradiation on the biochemical and biomechanical properties of sclera in a guinea pig model to control the progression of myopia. Experimental groups were administered 0.1% riboflavin solution with or without vitamin C by gavage from 3 days before myopic modeling and during the modeling process. Guinea pigs underwent 30 min of whole-body UVA irradiation after each gavage for 2 weeks. For control groups, guinea pigs were administered vitamin C and underwent either whole-body UVA irradiation without 0.1% riboflavin solution or whole-body fluorescent lamp irradiation with or without 0.1% riboflavin solution. Resultantly, myopia models were established with an increased axial length and myopic diopter. Compared with myopic eyes in the control groups, the net increase in axial length, diopter and strain assessment decreased significantly, and the net decrease in sclera thickness, ultimate load, and stress assessment decreased significantly in experimental groups. MMP-2 expression showed a lower net increase, while TIMP-2 expression showed a lower net decrease. In addition, hyperplasia of scleral fibroblasts was more active in myopic eyes of experimental groups. Overall, our results showed that oral administration of riboflavin with whole-body UVA irradiation could increase the strength and stiffness of sclera by altering the biochemical and biomechanical properties, and decreases in axial elongation and myopic diopter are greater in the guinea pig myopic model.
在本研究中，我们评估了口服核黄素联合全身紫外线A（UVA）照射对豚鼠模型巩膜生化和生物力学特性的影响，以控制近视的进展。实验组在近视建模前3天及建模过程中通过灌胃给予含或不含维生素C的0.1%核黄素溶液。每灌胃2周后，豚鼠接受30分钟的全身UVA照射。对照组豚鼠给予维生素C，并接受不含0.1%核黄素溶液的全身UVA照射或含或不含0.1%核黄素溶液的全身荧光灯照射。结果，建立了眼轴长度增加和近视屈光度增加的近视模型。与对照组的近视眼睛相比，实验组眼轴长度、屈光度和应变评估的净增加显著降低，巩膜厚度、极限负荷和应力评估的净降低也显著降低。基质金属蛋白酶-2（MMP-2）表达的净增加较低，而金属蛋白酶组织抑制因子-2（TIMP-2）表达的净降低较低。此外，实验组近视眼中巩膜成纤维细胞的增生更活跃。总体而言，我们的结果表明，口服核黄素联合全身UVA照射可通过改变生化和生物力学特性来增加巩膜的强度和硬度，并且在豚鼠近视模型中，眼轴伸长和近视屈光度的降低幅度更大。
