Institute of Biology, Komi Science Centre, Ural Division, Russian Academy of Sciences, Syktyvkar 167982, Russian Federation.
Institute of Chemistry, Komi Science Centre, Ural Division, Russian Academy of Sciences, Syktyvkar 167982, Russian Federation.
J Biotechnol. 2017 Oct 20;260:31-37. doi: 10.1016/j.jbiotec.2017.08.024. Epub 2017 Aug 31.
The complex sorbents based on hydrophobized starch, which contain oil-degrading microorganisms, have been proposed for effective sorption and utilization of petroleum-related pollutants. The sorbents were made on the base of benzoic, lauric and stearic acid esters of starch with degrees of substitution of 0.4-1.1. The esterification of starch was carried out by the reaction with acyl chlorides of the corresponding acids in an aqueous-organic medium. The structure of the esters was studied by SEM, IR and NMR spectroscopy. As a result of porous hydrophobic structure, these sorbents are capable of binding and retention of petroleum products on the water surface, and keeping the flotation for at least 30days after the petroleum products sorption. The test of biodegradability of the obtained samples revealed that the modified starches can be degraded by microscopic fungi, therefore they do not cause secondary pollution. The cultures of yeast Rhodotorula glutinis VKM Y-2993D and bacteria Pseudomonas libanensis VKM B-3041D immobilized on the sorbent facilitate the rapid utilization of accumulated petroleum products.
基于疏水化淀粉的复杂吸附剂，其中含有可降解油的微生物，已被提议用于有效吸附和利用与石油相关的污染物。这些吸附剂是在淀粉的苯甲酸酯、月桂酸酯和硬脂酸酯的基础上制成的，取代度为 0.4-1.1。淀粉的酯化反应是在水-有机介质中与相应酸的酰氯进行的。通过 SEM、IR 和 NMR 光谱研究了酯的结构。由于多孔疏水结构，这些吸附剂能够在水面上结合和保留石油产品，并在石油产品吸附后至少保持 30 天的浮选。对所得样品的可生物降解性的测试表明，改性淀粉可以被微观真菌降解，因此它们不会造成二次污染。固定在吸附剂上的酵母 Rhodotorula glutinis VKM Y-2993D 和细菌 Pseudomonas libanensis VKM B-3041D 的培养物有利于加速利用积累的石油产品。
