Yang Ling, Lu Xingmeng, Fang Weihuan
Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, 310058, China.
Biotechnol Lett. 2017 Dec;39(12):1821-1825. doi: 10.1007/s10529-017-2426-y. Epub 2017 Sep 1.
To develop a simple method for efficient expression of classical swine fever virus (CSFV) E2 protein.
The pFastBac HT B vector (pFastHTB-M1) was modified by adding a melittin signal peptide sequence. The E2 gene fragment without the transmembrane region was cloned into pFastHTB-M1. The modified vector has clear advantage over the original one, as evidenced by the purified recombinant E2 protein that was detected significantly by SDS-PAGE.
The modified vector has the potential for large-scale production and easy purification of the CSFV E2 protein or other proteins of interests.
开发一种高效表达经典猪瘟病毒（CSFV）E2蛋白的简单方法。
通过添加蜂毒素信号肽序列对pFastBac HT B载体（pFastHTB-M1）进行修饰。将不含跨膜区的E2基因片段克隆到pFastHTB-M1中。经SDS-PAGE检测，纯化的重组E2蛋白显示出明显优势，证明修饰后的载体比原始载体具有明显优势。
修饰后的载体具有大规模生产和易于纯化CSFV E2蛋白或其他感兴趣蛋白的潜力。
