Kondratyeva L G, Didych D A, Chernov I P, Kopantzev E P, Stukacheva E A, Vinogradova T V, Sverdlov E D
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
Dokl Biochem Biophys. 2017 Jul;475(1):259-263. doi: 10.1134/S1607672917040056. Epub 2017 Sep 2.
Exogenous expression of the gene encoding the pancreatic master regulator PDX1 in cell lines with different degrees of differentiation of pancreatic cancer cells is accompanied by changes in the expression of known master genes involved in cancer progression. In BxPC3 cells, as compared to BxPC3, we detected an increased expression of the following genes: NKX6.1 (2 times), NR5A2 (2.5 times), KLF5 (1.8 times), ZEB1 (3 times), and ONECUT1 (1.3 times), as well as a decreased expression of MUC1 and SLUG genes (3 and 2 times, respectively). In PANC1 cells, as compared to the control PANC1 cells, we detected a decreased expression of ISL1 (2 times) and an increased expressed of KRT8 (2 times) and MUC1 (by 30%). In the high-grade cell lines (including the BxPC3 line studied), the total content of sites containing the marks of active enhancers was higher than that in the low-grade cell lines (PANC1).
在具有不同分化程度的胰腺癌细胞系中，胰腺主调节因子PDX1编码基因的外源性表达伴随着参与癌症进展的已知主基因表达的变化。在BxPC3细胞中，与BxPC3相比，我们检测到以下基因表达增加：NKX6.1（2倍）、NR5A2（2.5倍）、KLF5（1.8倍）、ZEB1（3倍）和ONECUT1（1.3倍），以及MUC1和SLUG基因表达降低（分别为3倍和2倍）。在PANC1细胞中，与对照PANC1细胞相比，我们检测到ISL1表达降低（2倍），KRT8表达增加（2倍），MUC1表达增加（30%）。在高级别细胞系（包括所研究的BxPC3系）中，含有活性增强子标记的位点的总含量高于低级别细胞系（PANC1）。
