利用蛇α-神经毒素与量子点的缀合物检测人神经元α7烟碱型乙酰胆碱受体
Detection of human neuronal α7 nicotinic acetylcholine receptors by conjugates of snake α-neurotoxin with quantum dots.
作者信息
Makarova Ya V, Shelukhina I V, Mukherjee A K, Kuznetsov D V, Tsetlin V I, Utkin Yu N
机构信息
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
Tezpur University, Tezpur, India.
出版信息
Dokl Biochem Biophys. 2017 Jul;475(1):253-255. doi: 10.1134/S1607672917040032. Epub 2017 Sep 2.
Fluorescent derivatives are widely used to study the structure and functions of proteins. Quantum dots (QDs), fluorescent semiconductor nanocrystals, have a high quantum yield and are much more resistant to bleaching compared to organic dyes. Conjugates of α-neurotoxins with QDs were used for visualization of human α7 acetylcholine receptors heterologously expressed in GH4C1 pituitary adenoma cells. Specific staining of cells by the conjugated toxins was observed.
荧光衍生物被广泛用于研究蛋白质的结构和功能。量子点（QDs），即荧光半导体纳米晶体，具有高量子产率，与有机染料相比，其抗光漂白能力要强得多。α-神经毒素与量子点的缀合物被用于可视化在GH4C1垂体腺瘤细胞中异源表达的人类α7乙酰胆碱受体。观察到缀合毒素对细胞的特异性染色。
Zotero 插件