Fushuku N, Muramatsu H, Uezono M M, Muramatsu T
J Biol Chem. 1987 Jul 25;262(21):10086-92.
Culture fluid of Clostridium perfringens contained an endoglycosidase releasing a galactosyl disaccharide from glycans derived from glycoproteins of teratocarcinoma OTT6050. The endoglycosidase was purified by ammonium sulfate precipitation, Sephadex G-200 column chromatography, and DEAE-Sephadex A-25 column chromatography. The structure of the disaccharide product was determined to be Gal alpha 1----3Gal. The enzyme also released the disaccharide from bovine thyroglobulin glycopeptide and from a pentaglycosyl ceramide (Gal alpha 1----3Gal beta 1----4GlcNAc beta 1----3Gal beta 1----4Glc-Cer). Therefore, we concluded that the enzyme was an endo-beta-galactosidase cleaving Gal beta 1----4GlcNAc linkage. Keratan sulfate and the antigenic determinant of blood group type B were resistant to the enzyme: the specificity of the enzyme is different from other endo-beta-galactosidases so far described. The enzyme could act on intact thyroglobulin and defucosylated ovarian cyst mucin.
产气荚膜梭菌的培养液中含有一种内切糖苷酶，该酶可从源自畸胎瘤OTT6050糖蛋白的聚糖中释放出一种半乳糖基二糖。通过硫酸铵沉淀、Sephadex G - 200柱色谱和DEAE - Sephadex A - 25柱色谱对该内切糖苷酶进行了纯化。确定二糖产物的结构为Galα1----3Gal。该酶还能从牛甲状腺球蛋白糖肽和一种五糖基神经酰胺（Galα1----3Galβ1----4GlcNAcβ1----3Galβ1----4Glc - Cer）中释放出二糖。因此，我们得出结论，该酶是一种切割Galβ1----4GlcNAc键的内切β - 半乳糖苷酶。硫酸角质素和B血型抗原决定簇对该酶具有抗性：该酶的特异性与迄今描述的其他内切β - 半乳糖苷酶不同。该酶可作用于完整的甲状腺球蛋白和去岩藻糖基化的卵巢囊肿粘蛋白。
