CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.
Wuhan Institutes of Biotechnology, Wuhan, 430075, China.
Virol Sin. 2017 Dec;32(6):537-540. doi: 10.1007/s12250-017-4018-3.
In this study, we improved the most commonly used methods for MS detection of SUMOylated sites and used an E. coli recombination SUMOylation system with SUMO-1 (T95R). This system provides fast enrichment of SUMOylated viral protein in less than 2 days, and shows advantage over the method of collecting modified protein from cells in convenience and sensitivity. Furthermore, this method provides an option for rapid and accurate identification of the potential viral protein SUMOylation sites.
在这项研究中，我们改进了最常用的 SUMO 化位点 MS 检测方法，并使用了带有 SUMO-1（T95R）的大肠杆菌重组 SUMO 化系统。该系统可在不到 2 天的时间内快速富集 SUMO 化的病毒蛋白，与从细胞中收集修饰蛋白的方法相比，具有方便和灵敏的优势。此外，该方法为快速准确鉴定潜在的病毒蛋白 SUMO 化位点提供了一种选择。
