Cai Jianfeng, Xin Huaxia, Cheng Lingping, Fu YanHui, Jiang Dasen, Feng Jiatao, Fu Qing, Jin Yu, Liang Xinmiao
Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
Hai Nan Normal University, 99 South Longkun Road, Haikou, 571127, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 15;1063:149-155. doi: 10.1016/j.jchromb.2017.08.034. Epub 2017 Aug 31.
The goal of this study was to develop a method that utilized a hydrophilic C18 stationary phase in the preparative high performance liquid chromatography to isolate the polar part from the rhizomes of Anemarrhena asphodeloides. The results showed that an initial mobile phase of pure water for the separation could greatly increase the retention and solubility of the polar compounds at the preparative scale. Introducing polar groups on the surface of the hydrophilic C18 column together with the use of optimized mobile phase compositions improved the column separation selectivity for polar compounds. Eleven previously undescribed compounds in Anemarrhena asphodeloides were obtained, indicating that the method developed in this study would facilitate the purification and separation of the polar part of traditional Chinese medicines.
