Orthopaedic Institute, Department of Orthopaedics, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215006, PR China.
Orthopaedic Institute, Department of Orthopaedics, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215006, PR China; International Research Center for Translational Orthopaedics (IRCTO), Soochow University, Suzhou, Jiangsu 215006, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Nov 1;80:352-361. doi: 10.1016/j.msec.2017.05.070. Epub 2017 May 14.
To meet the requirements of minimally invasive surgical treatments for osteoporotic vertebral compression fracture, various strategies have been proposed to enhance the properties of calcium phosphate cement (CPC). Here, a new strategy was developed by incorporating poly (γ-glutamic acid) (γ-PGA) and its strontium salt into the formulation of alpha tricalcium phosphate (α-TCP)-based CPC. Effects of γ-PGA on the injectability, cohesion, setting times, mechanical compressive strengths and cytocompatibility were systematically studied. Results showed that the injectability, cohesion and setting times were considerably improved by introducing γ-PGA into the CPC. Moreover, after setting for 7days, the compressive strengths increased from 14.6±1.4MPa for pure CPC to 35.3±1.7MPa for CPC with 8.9wt% γ-PGA and further to 61.2±5.4MPa for CPC with 8.9wt% γ-PGA combined with its strontium salt. In vitro osteoblast proliferation tests showed that the group of CPC modified by γ-PGA and its strontium salt had better cytocompatibility than the pure CPC group. All results suggest that optimal properties were obtained for the cement with 8.9wt% γ-PGA added to its solid phases and using strontium salt as the reactive liquid phase, making it as a promising candidate for application in kyphoplasty.
为满足骨质疏松性椎体压缩性骨折微创治疗的要求，提出了各种增强磷酸钙水泥（CPC）性能的策略。在这里，我们将聚（γ-谷氨酸）（γ-PGA）及其锶盐掺入到以α-磷酸三钙（α-TCP）为基础的 CPC 配方中，开发了一种新的策略。系统研究了γ-PGA 对可注射性、内聚性、凝固时间、机械压缩强度和细胞相容性的影响。结果表明，通过将γ-PGA 引入 CPC，可以显著提高其可注射性、内聚性和凝固时间。此外，在 7 天凝固后，抗压强度从纯 CPC 的 14.6±1.4MPa 增加到添加 8.9wt%γ-PGA 的 CPC 的 35.3±1.7MPa，再增加到添加 8.9wt%γ-PGA 及其锶盐的 CPC 的 61.2±5.4MPa。体外成骨细胞增殖试验表明，用γ-PGA 和其锶盐改性的 CPC 组比纯 CPC 组具有更好的细胞相容性。所有结果均表明，当将 8.9wt%γ-PGA 添加到其固相并用锶盐作为反应液相时，水泥具有最佳性能，有望在经皮椎体成形术中得到应用。
