Department of Biomedical Engineering, Middle East Technical University, Ankara 06800, Turkey.
Department of Engineering Sciences, Middle East Technical University, Ankara 06800, Turkey.
Mater Sci Eng C Mater Biol Appl. 2017 Nov 1;80:484-493. doi: 10.1016/j.msec.2017.06.016. Epub 2017 Jun 19.
Guided bone regeneration (GBR) concept has been developed to prevent the formation of non-functional scar tissue layer on defect site by undertaking barrier role. In this study, a new bilayer membrane which consisted of one layer of electrospun silk fibroin/PCL-PEG-PCL incorporating nanocalcium phosphate (SPCA) and one layer of PCL membrane was developed for GBR. To improve the osteoconductivity of membranes, nanosized calcium phosphate particles synthesized by Flame Spray Pyrolysis method were incorporated into membranes at 10% (wt) (SPCA10) and 20% (wt) (SPCA20) of the polymer content. The structural and chemical analyses revealed the well-integrated two layers of membranes with a total thickness of ca 100μm. In the regenerative layer, the highly porous mesh structure had a thickness of 12.6μm with randomly oriented fibers having diameters around 760nm, and nanoparticles dispersed homogenously. The mechanical test results showed remarkable improvement on the tensile strength of membranes with incorporation of nanoparticles. Higher water affinity of nanoCaP included membranes was proved by lower contact angle values and higher percent water uptake capacity. Biomineralization assay revealed that nucleation and growth of apatites around fibers of SPCA10 and SPCA20 were apparent while on SPCA0 apatite minerals were barely detected after 10days. Human dental pulp stem cells (DPSC) were seeded on electrospun layer of the bilayer membranes for biocompatibility and osteo-compatibility study. Increasing nanoCaP amount resulted in higher cell adhesion, proliferation, ALP activity and calcium deposition on membranes. These overall results confirmed the biocompatibility and potential applicability of proposed membranes for GBR treatments.
引导骨再生（GBR）的概念是通过承担屏障作用来防止在缺陷部位形成非功能性的瘢痕组织层。在这项研究中，开发了一种由一层电纺丝纤维蛋白/PCL-PEG-PCL 纳米磷酸钙（SPCA）和一层 PCL 膜组成的双层膜，用于 GBR。为了提高膜的骨诱导性，将火焰喷雾热解法合成的纳米磷酸钙颗粒以聚合物含量的 10%（wt）（SPCA10）和 20%（wt）（SPCA20）掺入膜中。结构和化学分析表明，两层膜的结合良好，总厚度约为 100μm。在再生层中，高度多孔的网状结构厚度为 12.6μm，随机取向的纤维直径约为 760nm，纳米颗粒均匀分散。力学测试结果表明，纳米颗粒的掺入显著提高了膜的拉伸强度。含纳米 CaP 的膜具有更高的亲水性，表现为接触角值较低和吸水率较高。生物矿化试验表明，SPCA10 和 SPCA20 纤维周围的磷灰石的成核和生长明显，而在 SPCA0 上，磷灰石矿物在 10 天后几乎检测不到。将人牙髓干细胞（DPSC）接种到双层膜的电纺丝层上，以研究其生物相容性和成骨相容性。随着纳米 CaP 含量的增加，细胞在膜上的黏附、增殖、碱性磷酸酶活性和钙沉积都有所增加。这些结果总体上证实了所提出的膜用于 GBR 治疗的生物相容性和潜在适用性。
