Department of Thoracic Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing, 210009, China; The Fourth Clinical College of Nanjing Medical University, Nanjing, 210000, China.
The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210000, China.
Cancer Lett. 2017 Nov 1;408:112-120. doi: 10.1016/j.canlet.2017.08.030. Epub 2017 Sep 1.
Reversible methylation by means of N6-methyladenosine (mA) is the most prevalent internal modification in mammalian mRNA. This RNA chemical mark is created by proteins that are mA "writers" and can be reversed by proteins that are mA "erasers" (i.e., demethylases). Some other proteins serving as "readers" can recognize mA-containing mRNA and regulate downstream molecular mechanisms accordingly. Although mA bases in RNA perform critical functions in important biological processes, their roles in cancer biology and cancer stem cells remain largely unknown. In this review, we focus on the mA-associated mechanisms and modification landscapes in several major malignant tumors. Global and detailed analyses were both conducted on relevant high-throughput sequencing data. Possible interventions against mA demethylases are also explored in this review, which may be advantageous for the treatment of mA related cancers.
通过 N6-甲基腺嘌呤（mA）实现的可逆甲基化是哺乳动物 mRNA 中最普遍的内部修饰。这种 RNA 化学标记是由 mA“写入器”蛋白创建的，并且可以被 mA“橡皮擦”（即去甲基酶）蛋白逆转。一些其他作为“读取器”的蛋白质可以识别含有 mA 的 mRNA，并相应地调节下游分子机制。尽管 RNA 中的 mA 碱基在重要的生物过程中发挥着关键作用，但它们在癌症生物学和癌症干细胞中的作用在很大程度上仍是未知的。在这篇综述中，我们重点介绍了几种主要恶性肿瘤中与 mA 相关的机制和修饰图谱。对相关高通量测序数据进行了全局和详细的分析。本文还探讨了针对 mA 去甲基酶的可能干预措施，这可能有利于治疗与 mA 相关的癌症。
