College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China.
College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China.
Microb Pathog. 2017 Oct;111:203-211. doi: 10.1016/j.micpath.2017.08.035. Epub 2017 Sep 1.
We analyzed here the complete genome sequences of a highly virulent Flavobacterium columnare Pf1 strain isolated in our laboratory. The complete genome consists of a 3,171,081 bp circular DNA with 2784 predicted protein-coding genes. Among these, 286 genes were predicted as antibiotic resistance genes, including 32 RND-type efflux pump related genes which were associated with the export of aminoglycosides, indicating inducible aminoglycosides resistances in F. columnare. On the other hand, 328 genes were predicted as pathogenicity related genes which could be classified as virulence factors, gliding motility proteins, adhesins, and many putative secreted proteases. These genes were probably involved in the colonization, invasion and destruction of fish tissues during the infection of F. columnare. Apparently, our obtained complete genome sequences provide the basis for the explanation of the interactions between the F. columnare and the infected fish. The predicted antibiotic resistance and pathogenicity related genes will shed a new light on the development of more efficient preventional strategies against the infection of F. columnare, which is a major worldwide fish pathogen.
我们在这里分析了一株高度毒力的柱状黄杆菌 Pf1 菌株的全基因组序列，该菌株是我们实验室分离的。完整的基因组由一个 3,171,081 bp 的环状 DNA 组成，包含 2784 个预测的蛋白编码基因。其中，286 个基因被预测为抗生素耐药基因，包括 32 个与氨基糖苷类药物外排有关的 RND 型外排泵相关基因，表明柱状黄杆菌存在诱导型氨基糖苷类耐药性。另一方面，328 个基因被预测为与致病性相关的基因，可分为毒力因子、滑行运动蛋白、黏附素和许多假定的分泌蛋白酶。这些基因可能参与了柱状黄杆菌感染鱼类组织时的定植、入侵和破坏。显然，我们获得的完整基因组序列为解释柱状黄杆菌与感染鱼类之间的相互作用提供了基础。预测的抗生素耐药性和致病性相关基因将为开发更有效的预防柱状黄杆菌感染的策略提供新的思路，因为柱状黄杆菌是一种全球性的鱼类病原体。
