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内含子长度对小鼠μ重链mRNA差异加工的影响。

Effects of intron length on differential processing of mouse mu heavy-chain mRNA.

作者信息

Tsurushita N, Korn L J

出版信息

Mol Cell Biol. 1987 Jul;7(7):2602-5. doi: 10.1128/mcb.7.7.2602-2605.1987.

DOI:10.1128/mcb.7.7.2602-2605.1987
PMID:2886909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365397/
Abstract

Production of membrane-bound and secreted forms of mouse mu heavy-chain mRNA is controlled by differential processing in a developmental-stage-specific manner. We have analyzed the effects of various deletions and insertions in the C4-M1 intron of the mouse mu gene on the differential processing of mu mRNA. We show that there is a correlation between the length of the C4-M1 intron and the molar ratio of membrane-bound to secreted mu mRNAs, i.e., the shorter the C4-M1 intron, the higher the ratio. Since the poly(A) addition signal in the C4-M1 intron seems to be intact in the mutant mu genes, it is likely that the efficiency of splicing of the C4-M1 intron is affected by changes in the intron length.

摘要

小鼠μ重链mRNA的膜结合形式和分泌形式的产生是由发育阶段特异性的差异加工所控制的。我们分析了小鼠μ基因C4-M1内含子中各种缺失和插入对μ mRNA差异加工的影响。我们发现C4-M1内含子的长度与膜结合型和分泌型μ mRNA的摩尔比之间存在相关性，即C4-M1内含子越短，该比例越高。由于突变的μ基因中C4-M1内含子的聚腺苷酸化信号似乎是完整的，因此C4-M1内含子的剪接效率很可能受到内含子长度变化的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b951/365397/01e4e5b462db/molcellb00079-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b951/365397/5702b3955043/molcellb00079-0313-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b951/365397/01e4e5b462db/molcellb00079-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b951/365397/5702b3955043/molcellb00079-0313-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b951/365397/01e4e5b462db/molcellb00079-0314-a.jpg

相似文献

1
Effects of intron length on differential processing of mouse mu heavy-chain mRNA.内含子长度对小鼠μ重链mRNA差异加工的影响。
Mol Cell Biol. 1987 Jul;7(7):2602-5. doi: 10.1128/mcb.7.7.2602-2605.1987.
2
Regulation of differential processing of mouse immunoglobulin mu heavy-chain mRNA.小鼠免疫球蛋白μ重链mRNA差异加工的调控
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3
The regulated production of mu m and mu s mRNA is dependent on the relative efficiencies of mu s poly(A) site usage and the c mu 4-to-M1 splice.μm和μs mRNA的调控产生取决于μs多聚腺苷酸化位点使用效率和cμ4到M1剪接的相对效率。
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4
Regulated production of mu m and mu s mRNA requires linkage of the poly(A) addition sites and is dependent on the length of the mu s-mu m intron.μm和μs mRNA的调控产生需要多聚腺苷酸化位点的连接，并且依赖于μs-μm内含子的长度。
Proc Natl Acad Sci U S A. 1986 Dec;83(23):8883-7. doi: 10.1073/pnas.83.23.8883.
5
Balanced efficiencies of splicing and cleavage-polyadenylation are required for mu-s and mu-m mRNA regulation.μ-s和μ-m mRNA调控需要剪接与切割-聚腺苷酸化的平衡效率。
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6
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7
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Role of an RNA cleavage/poly(A) addition site in the production of membrane-bound and secreted IgM mRNA.RNA 切割/聚腺苷酸化位点在膜结合型和分泌型 IgM mRNA 产生中的作用。
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9
Poly(A) site choice rather than splice site choice governs the regulated production of IgM heavy-chain RNAs.聚腺苷酸化位点的选择而非剪接位点的选择决定了IgM重链RNA的调控产生。
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Nuclear factors in B lymphoma enhance splicing of mouse membrane-bound mu mRNA in Xenopus oocytes.B淋巴瘤中的核因子增强非洲爪蟾卵母细胞中小鼠膜结合型μ mRNA的剪接。
Science. 1988 Jan 29;239(4839):494-7. doi: 10.1126/science.3124268.

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本文引用的文献

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Establishment of B cell hybridomas with B cell surface antigens.利用B细胞表面抗原建立B细胞杂交瘤。
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