College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China.
Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Bioresour Technol. 2017 Nov;244(Pt 1):1166-1172. doi: 10.1016/j.biortech.2017.08.046. Epub 2017 Aug 12.
The surface characterization and electrostatic property of Hybrid Pennisetum (HP) after alkaline sulfite pretreatment were explored for enhanced enzymatic hydrolysability. The O/C ratio in HP increased from 0.34 to 0.60, and C1 concentration decreased from 62.5% to 31.6%, indicating that alkaline sulfite pretreatment caused poorer lignin but richer carbohydrate on HP surface. Zeta potential and sulfur element analysis indicated that more enzymes would preferably adsorb on the carbohydrate surface of alkaline sulfite pretreated HP because the lignin was sulfonated, which facilitated the decrease of non-productive adsorption. Glucose yield of alkaline sulfite pretreated HP reached to 100% by synergistic action of cellulase and xylanase in the hydrolysis, which was significantly higher than that of NaOH pretreated, and the concentration of glucose released was 1.52times higher. The results suggested that alkaline sulfite pretreatment had potential for improving the HP hydrolysability, and the surface characterization and electrostatic property facilitated the enzymatic digestibility.
探讨了经碱性亚硫酸盐预处理后的杂交狼尾草（HP）的表面特性和静电特性，以提高其酶水解性能。HP 的 O/C 比从 0.34 增加到 0.60，C1 浓度从 62.5%降低到 31.6%，表明碱性亚硫酸盐预处理导致 HP 表面木质素减少而碳水化合物增加。动电位和硫元素分析表明，由于木质素磺化，更多的酶会优先吸附在碱性亚硫酸盐预处理 HP 的碳水化合物表面，从而减少非生产性吸附。碱性亚硫酸盐预处理 HP 在纤维素酶和木聚糖酶的协同作用下水解得到 100%的葡萄糖产率，明显高于氢氧化钠预处理，释放的葡萄糖浓度提高了 1.52 倍。结果表明，碱性亚硫酸盐预处理具有提高 HP 水解性能的潜力，表面特性和静电特性有助于酶解。
