Uma Maheshwari S, Govindan K, Raja M, Raja A, Pravin M B S, Vasanth Kumar S
Department of Physics, Mother Teresa Women's University, Kodaikanal - 624 101, Tamil Nadu, India.
Department of Nanoscience and Nanotechnology, Karunya University, Coimbatore, India.
Biomed Mater Eng. 2017;28(4):401-415. doi: 10.3233/BME-171682.
Polyvinylalcohol (PVA) and Polyvinylpyrrolidone (PVP) blend incorporated with hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) is electrospun as nanofibrous composite scaffolds to act as suitable template for bone tissue engineering. Microscopic and spectroscopic characterizations confirm uniform integration of the crystalline calcium phosphate ceramics in the scaffolds. PVA-PVP blends are usually amorphous in nature and addition of phosphate ceramic particles specifically HAp elevates its crystalline behavior which is substantiated by XRD details. Further incorporation of ceramics is confirmed using FT-IR as characteristic PO43- groups for HAp and β-TCP were observed in the distinguished composites. Single glass transition temperature is observed for pure and composite blends indicating the formation of highly miscible blends. Also, addition of these ceramics augments the thermal stability of the blend scaffolds. Biocompatibility of the prepared (PVA: PVP)-HAp and (PVA: PVP)-TCP scaffolds is assessed using MG-63 Osteoblast cell lines in the time interval of 1st, 4th and 7th day. The cell viability percentage for (PVA: PVP)-HAp is high compared to β-TCP added blend composites, reinforcing the fact that scaffolds with good mechanical strength and enhanced porosity supports better cell adhesion.
将与羟基磷灰石（HAp）和β - 磷酸三钙（β - TCP）混合的聚乙烯醇（PVA）和聚乙烯吡咯烷酮（PVP）进行静电纺丝，制成纳米纤维复合支架，作为骨组织工程的合适模板。微观和光谱表征证实了结晶磷酸钙陶瓷在支架中的均匀整合。PVA - PVP共混物本质上通常是无定形的，添加磷酸陶瓷颗粒，特别是HAp，提高了其结晶行为，XRD细节证实了这一点。通过FT - IR进一步证实了陶瓷的掺入，因为在不同的复合材料中观察到了HAp和β - TCP的特征性PO43 - 基团。纯共混物和复合共混物均观察到单一玻璃化转变温度，表明形成了高度互溶的共混物。此外，添加这些陶瓷增强了共混支架的热稳定性。使用MG - 63成骨细胞系在第1、4和7天的时间间隔内评估制备的（PVA：PVP） - HAp和（PVA：PVP） - TCP支架的生物相容性。与添加β - TCP的共混复合材料相比，（PVA：PVP） - HAp的细胞活力百分比更高，这强化了具有良好机械强度和增强孔隙率的支架支持更好细胞粘附的事实。
