Lea R W, Hall T R, Vowles D M
Neuroscience. 1987 May;21(2):639-45. doi: 10.1016/0306-4522(87)90149-7.
A method for measuring the concentration and turnover of serotonin, dopamine and noradrenaline in small amounts of brain tissue was validated for the ring dove (Streptopelia risoria). Turnover rates of the catecholamines calculated as the rate of depletion after tyrosine hydroxylase inhibition agreed well with turnover rate measured as rate of accumulation after inhibition of monoamine oxidase at 2.5 h, but not 5 h, after drug administration. Using the monoamine oxidase inhibitor, pargyline, turnover of serotonin, dopamine and noradrenaline were estimated in the same tissue. Both the concentration and turnover of the amines in hypothalamus, paleostriatum and hyperstriatum fluctuated over a 24 h period. Serotonin turnover was greatest during the middle of the dark period in all three tissues. In contrast, for both dopamine and noradrenaline the highest turnover was observed during the light period and the lowest during the dark period.
一种用于测量少量脑组织中血清素、多巴胺和去甲肾上腺素浓度及周转率的方法在斑鸠（Streptopelia risoria）中得到验证。以酪氨酸羟化酶抑制后耗竭率计算的儿茶酚胺周转率，与在给药后2.5小时而非5小时抑制单胺氧化酶后以积累率测量的周转率非常吻合。使用单胺氧化酶抑制剂优降宁，对同一组织中血清素、多巴胺和去甲肾上腺素的周转率进行了估计。下丘脑、古纹状体和新纹状体中胺类的浓度和周转率在24小时内波动。在所有三个组织中，血清素周转率在黑暗期中期最高。相比之下，多巴胺和去甲肾上腺素的周转率在光照期最高，在黑暗期最低。
