Department of Analytical Chemistry, Medical University of Lublin, Lublin, Poland.
Chair and Department of Biophysics, Medical University of Lublin, Lublin, Poland.
J Sep Sci. 2017 Nov;40(21):4176-4190. doi: 10.1002/jssc.201700748. Epub 2017 Oct 4.
Basic thermodynamic functions responsible for retention of new 1,2,4-triazole derivatives exhibiting varied antiepileptic activity on cholesterol-based stationary phase were determined. Evaluation of the Gibbs energy change, the change in enthalpy and the change in entropy was based on the van't Hoff relationship representing lnk versus 1/T. A detailed discussion of the van't Hoff equation, exploring the influence of the phase ratio on deviations from linearity in a van't Hoff plot is presented. We show chromatographic evidence to the question of how a varied mobile phase composition may cause different thermodynamic phase ratios. The analysis of data from a differential scanning calorimetry excluded any phase transitions of either the individual solutes or cholesterol stationary phase suspended in the mobile phase components within the studied temperature range.
确定了在基于胆固醇的固定相上表现出不同抗癫痫活性的新型 1,2,4-三唑衍生物保留的基本热力学函数。吉布斯自由能变化、焓变和熵变的评估是基于表示 lnk 与 1/T 的范特霍夫关系。本文详细讨论了范特霍夫方程，探讨了相比率对范特霍夫图中偏离线性的影响。我们展示了色谱证据，说明变化的流动相组成如何导致不同的热力学相比率。差示扫描量热法数据分析排除了在所研究的温度范围内，单个溶质或悬浮在流动相成分中的胆固醇固定相的任何相转变。
