Du Q, Yu X J, Li H J, Guan S Q, Zhang Z Y, Mei Y F
Department of Rheumatology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Zhonghua Nei Ke Za Zhi. 2017 Sep 1;56(9):655-659. doi: 10.3760/cma.j.issn.0578-1426.2017.09.007.
To assess the expression and significance of proprotein convertase subtilisin kexin 9 (PCSK9) in patients with rheumatoid arthritis (RA). Sixty-five RA patients and forty-seven healthy controls were recruited in this study. The body mass index (BMI) and serum total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL), lipoprotein a, low density lipoprotein(LDL), very low density lipoprotein(VLDL), apolipoprotein A(ApoA), apolipoprotein B(ApoB) and the ratio of LDL-C/HDL-C were tested. Other parameters included disease activity score 28(DAS28), rheumatoid factor (RF), anti-cyclic citrullinated peptide (CCP) antibody, erythrocyte sedimentation rate (ESR), c-reactive protein (CRP). Serum PCSK9 level was measured by ELISA and compared between RA patients and healthy controls. (1) The serum PCSK9 levels in RA patients were higher than those in healthy controls[(409.36±223.52) μg/L vs (292.19±109.79) μg/L, <0.05]. (2) Compared with subgroup of moderate and low active disease and patients in remission, PCSK9 was significantly higher in patients with highly active disease (<0.05). (3) The serum PCSK9 levels were positively correlated with RF, TC, TG, LDL, very low density lipoprotein (VLDL), ApoB, with values as 0.303, 0.490, 0.320, 0.451, 0.319, 0.463, respectively (<0.05). (4) Multiple stepwise regression analysis showed that DAS28, RF, TC and LDL-C/HDL-C were relevant factors for PCSK9 in RA patients. The serum PCSK9 level is elevated in RA patients, which is related to RF, disease activity, TC, TG, LDL, VLDL, ApoB. This suggests that PCSK9 is potentially linked to inflammatory reaction and lipid metabolism in rheumatoid arthritis.
评估前蛋白转化酶枯草溶菌素9（PCSK9）在类风湿关节炎（RA）患者中的表达及意义。本研究招募了65例RA患者和47例健康对照者。检测了体重指数（BMI）以及血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白（HDL）、脂蛋白a、低密度脂蛋白（LDL）、极低密度脂蛋白（VLDL）、载脂蛋白A（ApoA）、载脂蛋白B（ApoB）以及LDL-C/HDL-C比值。其他参数包括疾病活动度评分28（DAS28）、类风湿因子（RF）、抗环瓜氨酸肽（CCP）抗体、红细胞沉降率（ESR）、C反应蛋白（CRP）。采用酶联免疫吸附测定法（ELISA）检测血清PCSK9水平，并在RA患者和健康对照者之间进行比较。（1）RA患者血清PCSK9水平高于健康对照者[（409.36±223.52）μg/L对（292.19±109.79）μg/L，P<0.05]。（2）与中度和低度活动疾病亚组以及缓解期患者相比，高度活动疾病患者的PCSK9水平显著更高（P<0.05）。（3）血清PCSK9水平与RF、TC、TG、LDL、极低密度脂蛋白（VLDL）、ApoB呈正相关，相关系数分别为0.303、0.490、0.320、0.451、0.319、0.463（P<0.05）。（4）多元逐步回归分析显示，DAS28、RF、TC和LDL-C/HDL-C是RA患者PCSK9的相关影响因素。RA患者血清PCSK9水平升高，这与RF、疾病活动度、TC、TG、LDL、VLDL、ApoB有关。这表明PCSK9可能与类风湿关节炎中的炎症反应和脂质代谢相关。
