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硫化氢减轻异氟烷诱导的发育中大鼠大脑神经细胞凋亡及认知障碍。

Hydrogen sulfide attenuates isoflurane-induced neuroapoptosis and cognitive impairment in the developing rat brain.

作者信息

Hu Xueyuan, Luan Li, Guan Wei, Zhang Shuai, Li Bei, Ji Wei, Fan Honggang

机构信息

Department of Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, No. 600 of Changjiang Road, Xiangfang District, Harbin, Heilongjiang Province, China.

出版信息

BMC Anesthesiol. 2017 Sep 5;17(1):123. doi: 10.1186/s12871-017-0419-y.

DOI:10.1186/s12871-017-0419-y
PMID:28870150
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5584335/
Abstract

BACKGROUND

Isoflurane-induced neuroapoptosis and cognitive impairment has been previously reported. Hydrogen sulfide (HS) has been shown to be a neuromodulator that is thought to have anti-apoptotic, anti-inflammatory, and anti-oxidative benefits. However, it is not known if HS is protective against anesthesia-induced apoptosis and cognitive defects.

METHODS

In this study, postnatal day 7 (P7) Sprague-Dawley rats were randomly divided into four groups: control group (normal saline), HS group (NaHS 28 μM/kg), isoflurane group (normal saline +0.75% isoflurane) and HS preconditioning group (NaHS 28 μM/kg + 0.75% isoflurane). After exposure to isoflurane for 6 h, half the numbers of rats in each group were euthanized, and the hippocampus and cerebral cortex were dissected and examined for apoptosis by the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) technique and western blot. After 6 weeks, the remaining rats were subjected to a Morris water maze (MWM) test for behavioral assessment.

RESULTS

The TUNEL assay and western blot showed that when rats were preconditioned with NaHS, neuroapoptosis decreased significantly both in hippocampus and cerebral cortex compering with the isofulrane group. The MWM showed that P7 rats administration of NaHS improved cognitive impairments induced by isoflurane.

CONCLUSIONS

The current study demonstrates that HS attenuates isoflurane-induced neuroapoptosis and improves cognitive impairments in the developing rat brain.

摘要

背景

先前已有异氟烷诱导神经细胞凋亡和认知功能障碍的报道。硫化氢（HS）已被证明是一种神经调节剂，被认为具有抗凋亡、抗炎和抗氧化作用。然而，目前尚不清楚HS是否能预防麻醉诱导的细胞凋亡和认知缺陷。

方法

在本研究中，将出生后第7天（P7）的Sprague-Dawley大鼠随机分为四组：对照组（生理盐水）、HS组（硫氢化钠28 μM/kg）、异氟烷组（生理盐水+0.75%异氟烷）和HS预处理组（硫氢化钠28 μM/kg + 0.75%异氟烷）。暴露于异氟烷6小时后，每组半数大鼠安乐死，解剖海马体和大脑皮层，采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）技术和蛋白质免疫印迹法检测细胞凋亡情况。6周后，对其余大鼠进行莫里斯水迷宫（MWM）试验以进行行为评估。

结果

TUNEL检测和蛋白质免疫印迹显示，用硫氢化钠预处理大鼠时，与异氟烷组相比，海马体和大脑皮层的神经细胞凋亡均显著减少。莫里斯水迷宫试验表明，给P7大鼠注射硫氢化钠可改善异氟烷诱导的认知障碍。

结论

当前研究表明，HS可减轻异氟烷诱导的神经细胞凋亡，并改善发育中大鼠大脑的认知障碍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75d8/5584335/d402a8feeb82/12871_2017_419_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75d8/5584335/23b49cb5a013/12871_2017_419_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75d8/5584335/20835a941417/12871_2017_419_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75d8/5584335/d402a8feeb82/12871_2017_419_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75d8/5584335/23b49cb5a013/12871_2017_419_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75d8/5584335/20835a941417/12871_2017_419_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75d8/5584335/d402a8feeb82/12871_2017_419_Fig3_HTML.jpg

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本文引用的文献

1
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Handb Exp Pharmacol. 2015;230:193-215. doi: 10.1007/978-3-319-18144-8_10.
2
The role of hydrogen sulfide in pathologies of the vital organs and its clinical application.硫化氢在重要器官病变中的作用及其临床应用。
J Physiol Pharmacol. 2015 Apr;66(2):169-79.
3
Hydrogen sulfide-based therapeutics: exploiting a unique but ubiquitous gasotransmitter.基于硫化氢的治疗方法：利用一种独特而普遍存在的气体递质。
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Naunyn Schmiedebergs Arch Pharmacol. 2021 Jul;394(7):1553-1567. doi: 10.1007/s00210-021-02077-3. Epub 2021 Mar 27.
4
Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus.硫化氢通过上调海马体中的Sirt1来预防睡眠剥夺诱导的海马体损伤。
Front Neurosci. 2020 Mar 11;14:169. doi: 10.3389/fnins.2020.00169. eCollection 2020.
5
Neurotoxic Agent-Induced Injury in Neurodegenerative Disease Model: Focus on Involvement of Glutamate Receptors.神经退行性疾病模型中神经毒性剂诱导的损伤：聚焦谷氨酸受体的作用
Front Mol Neurosci. 2018 Aug 29;11:307. doi: 10.3389/fnmol.2018.00307. eCollection 2018.
Nat Rev Drug Discov. 2015 May;14(5):329-45. doi: 10.1038/nrd4433. Epub 2015 Apr 7.
4
Value of water mazes for assessing spatial and egocentric learning and memory in rodent basic research and regulatory studies.水迷宫在啮齿动物基础研究和监管研究中评估空间和自我中心学习与记忆的价值。
Neurotoxicol Teratol. 2014 Sep-Oct;45:75-90. doi: 10.1016/j.ntt.2014.07.003. Epub 2014 Aug 10.
5
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6
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7
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Behav Brain Res. 2014 Aug 1;269:6-14. doi: 10.1016/j.bbr.2014.04.027. Epub 2014 Apr 23.
8
H2S during circulatory shock: some unresolved questions.循环性休克中的硫化氢：一些未解决的问题。
Nitric Oxide. 2014 Sep 15;41:48-61. doi: 10.1016/j.niox.2014.03.163. Epub 2014 Mar 18.
9
Hydrogen sulfide slows down progression of experimental Alzheimer's disease by targeting multiple pathophysiological mechanisms.硫化氢通过靶向多种病理生理机制减缓实验性阿尔茨海默病的进展。
Neurobiol Learn Mem. 2013 Sep;104:82-91. doi: 10.1016/j.nlm.2013.05.006. Epub 2013 May 31.
10
Anaesthetic neurotoxicity and neuroplasticity: an expert group report and statement based on the BJA Salzburg Seminar.麻醉神经毒性和神经可塑性：基于 BJA 萨尔茨堡研讨会的专家组报告和声明。
Br J Anaesth. 2013 Aug;111(2):143-51. doi: 10.1093/bja/aet177. Epub 2013 May 30.