Subramanian Anuradha, Budhiraja Gaurav, Sahu Neety
Dept. of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
Dept. of Chemical and Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
Int J Biochem Cell Biol. 2017 Oct;91(Pt A):60-64. doi: 10.1016/j.biocel.2017.08.018. Epub 2017 Sep 10.
The primary cilium has been implicated in mechanotransduction, however, its mechanosensory role in transducing signals under low-intensity ultrasound (LIUS) which has the potential to repair fractures and cartilage, remains uninvestigated. This study examined the impact of continuous-wave US, at the cell resonance frequency of 5.0MHz and pressure amplitude of either 14 or 60kPa, on the incidence, length and orientation of primary cilium in bovine articular chondrocytes. Visualization of primary cilium with acetylated α-tubulin staining demonstrated that the primary cilium was elongated, bent under US and these changes were reversible. Basal expression of phospho-ERK1/2 was lower in deciliated chondrocytes, thus implicating the role for the primary cilium in transducing signals via the MAPK/ERK pathway. This study demonstrates that the chondrocyte primary cilium is mechanosensitive and responds to US by altering its length and orientation.
初级纤毛与机械转导有关，然而，其在低强度超声（LIUS）下转导信号的机械感觉作用仍未得到研究，而LIUS具有修复骨折和软骨的潜力。本研究考察了在5.0MHz细胞共振频率以及14kPa或60kPa压力幅值的连续波超声作用下，牛关节软骨细胞初级纤毛的发生率、长度和方向所受的影响。用乙酰化α-微管蛋白染色对初级纤毛进行可视化显示，初级纤毛会伸长，在超声作用下弯曲，且这些变化是可逆的。去纤毛软骨细胞中磷酸化ERK1/2的基础表达较低，因此表明初级纤毛在通过MAPK/ERK途径转导信号中发挥作用。本研究表明，软骨细胞初级纤毛具有机械敏感性，并通过改变其长度和方向对超声作出反应。
