Chen Zaijie, Cheng Qianqian, Hu Chanquan, Guo Xinrui, Chen Ziqiang, Lin Yan, Hu Taijiao, Bellizzi Maria, Lu Guodong, Wang Guo-Liang, Wang Zonghua, Chen Songbiao, Wang Feng
Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry UniversityFuzhou, China.
Biotechnology Research Institute, Fujian Academy of Agricultural SciencesFuzhou, China.
Front Plant Sci. 2017 Aug 21;8:1447. doi: 10.3389/fpls.2017.01447. eCollection 2017.
Inducible gene expression has emerged as a powerful tool for plant functional genomics. The estrogen receptor-based, chemical-inducible system XVE has been used in many plant species, but the limited systemic movement of inducer β-estradiol in transgenic rice plants has prohibited a wide use of the XVE system in this important food crop. Here, we constructed an improved chemical-regulated, site-specific recombination system by employing the XVE transactivator in combination with a Cre/-FRT system, and optimized a seed-soaking procedure for XVE induction in rice. By using a gene and an hpRNAi cassette targeted for as reporters, we demonstrated that soaking transgenic seeds with estradiol solution could induce highly efficient site-specific recombination in germinating embryos, resulting in constitutive and high-level expression of target gene or RNAi cassette in intact rice plants from induced seeds. The strategy reported here thereby provides a useful gene activation approach for effectively regulating gene expression in rice.
诱导型基因表达已成为植物功能基因组学的一种强大工具。基于雌激素受体的化学诱导系统XVE已在许多植物物种中使用，但诱导剂β-雌二醇在转基因水稻植株中的系统移动有限，这限制了XVE系统在这种重要粮食作物中的广泛应用。在此，我们通过将XVE反式激活因子与Cre/FRT系统结合，构建了一种改进的化学调控、位点特异性重组系统，并优化了水稻中XVE诱导的浸种程序。通过使用一个针对[具体基因]的基因和一个hpRNAi盒作为报告基因，我们证明用雌二醇溶液浸泡转基因种子可在萌发的胚中诱导高效的位点特异性重组，从而使诱导种子发育成的完整水稻植株中靶基因或RNAi盒组成型且高水平表达。因此，本文报道的策略为有效调控水稻基因表达提供了一种有用的基因激活方法。
