Pathak Pradeep Kumar, Gupta Kapuganti Jagadis
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, PO Box 10531, 10067, New Delhi, India.
Methods Mol Biol. 2017;1670:63-69. doi: 10.1007/978-1-4939-7292-0_7.
Pathogen infection leads to induction of defense responses which includes modulation of gene expression and changes in metabolism plants. Despite of extensive research little is information known about the role of respiration and photosynthesis during pathogen infection in plants. Limited methods are available to measure oxygen dynamics in response to pathogen infection. Here by using an oxygen microsensor we measured oxygen changes in tomato plants infected with avirulent Pseudomonas syringae pv. tomato DC3000. In this method plant is placed in a closed chamber and change in oxygen levels can be measured by an oxygen sensor.
病原体感染会引发植物的防御反应,其中包括基因表达的调控和植物新陈代谢的变化。尽管进行了广泛的研究,但关于病原体感染期间植物呼吸作用和光合作用的作用仍知之甚少。目前用于测量病原体感染后氧气动态变化的方法有限。在此,我们使用氧微传感器测量了感染无毒丁香假单胞菌番茄致病变种DC3000的番茄植株中的氧气变化。在该方法中,将植物置于密闭室内,氧气水平的变化可通过氧传感器进行测量。