线粒体呼吸与氧张力

Mitochondrial Respiration and Oxygen Tension.

作者信息

Shaw Daniel S, Meitha Karlia, Considine Michael J, Foyer Christine H

机构信息

Centre for Plant Science, School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.

The UWA Institute of Agriculture, and School of Molecular Sciences, University of Western Australia, Perth, 6009, Australia.

出版信息

Methods Mol Biol. 2017;1670:97-113. doi: 10.1007/978-1-4939-7292-0_11.

DOI:10.1007/978-1-4939-7292-0_11

PMID:28871539

Abstract

Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO production and O consumption that enables calculation of the respiratory quotient [CO]/[O].

摘要

对植物器官的呼吸作用和氧张力进行测量，有助于在细胞氧代谢的背景下精确了解线粒体的能力和功能。在此，我们描述了可常规用于分离完整线粒体、测定呼吸电子传递的方法，以及体内测定氧张力和通过测量二氧化碳产生量和氧气消耗量来测定呼吸作用的技术，这些技术能够计算呼吸商[CO₂]/[O₂]。