State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, China.
College of Environment and Planning, Henan University, Kaifeng, China.
Microbiologyopen. 2017 Oct;6(5). doi: 10.1002/mbo3.503. Epub 2017 Sep 4.
Sediment microbial communities play an important role in lake trophic status. This study determined millions of Illumina reads (16S rRNA gene amplicons) to compare the bacterial communities in moderately eutrophic, lightly eutrophic, and moderately trophic regions using a technically consistent approach. The results indicated that the sediments from moderately eutrophic and trophic lake had the higher bacterial diversity than lightly eutrophic lake. Proteobacteria was the most abundant phylum (22.7%-86.2%) across samples from three regions. The sediments from moderately eutrophic region were enriched with Chloroflexi and Nitrospirae. Alphaproteobacteria, Gammaproteobacteria, and Bacteroidetes were enriched in the sediments from lightly eutrophic lake. The sediments from moderately trophic lake contained a high abundance of Acidobacteria and Deltaproteobacteria because of the low pH of the sediments in this lake. In moderately eutrophic region, Nitrospira held an absolute predominance, while Lysobacter and Flavobacterium were the most predominant genera in lightly eutrophic region. Temperature was the main factor influencing the bacterial community in the three lakes. The bacterial communities in the sediment samples obtained from moderately eutrophic lake were associated with nutrient concentration, whereas organic matter and total nitrogen contents mainly influenced the bacterial communities in sediments obtained from lightly eutrophic lake and moderately trophic lake, respectively.
沉积物微生物群落对湖泊营养状态起着重要作用。本研究采用技术一致的方法，通过比较中度富营养化、轻度富营养化和中度营养化区域的细菌群落，确定了数百万个 Illumina 读取（16S rRNA 基因扩增子）。结果表明，中度富营养化和富营养化湖泊的沉积物具有比轻度富营养化湖泊更高的细菌多样性。在三个区域的样本中，变形菌门是最丰富的门（22.7%-86.2%）。中度富营养化区域的沉积物富含绿弯菌门和硝化螺旋菌门。在轻度富营养化湖泊的沉积物中，α变形菌门、γ变形菌门和拟杆菌门得到了富集。由于该湖泊沉积物的 pH 值较低，富含酸杆菌门和脱铁杆菌门。在中度富营养化区域，硝化螺旋菌占据绝对优势，而赖氏菌属和黄杆菌属是轻度富营养化区域最主要的属。温度是影响三个湖泊中细菌群落的主要因素。从中度富营养化湖泊中获得的沉积物样本中的细菌群落与营养浓度有关，而有机质和总氮含量分别主要影响从轻度富营养化湖泊和中度富营养化湖泊中获得的沉积物中的细菌群落。
