a Department of Biomedical Science, Faculty of Medicine and Health Sciences , Universiti Putra Malaysia , Serdang , Selangor , Malaysia.
b Department of Pathology, Faculty of Medicine and Health Sciences , Universiti Putra Malaysia , Serdang , Selangor , Malaysia.
Pharm Biol. 2017 Dec;55(1):2102-2109. doi: 10.1080/13880209.2017.1371769.
Leaves of Muntingia calabura (Elaeocarpaceae) are widely used in traditional medical practice; scientific findings show various pharmacological activities. However, its anticancer effect has not been investigated thoroughly yet.
The objective of this study is to study the chemoprevention effects of MEMC against azoxymethane (AOM)-induced colon cancer and to examine the involvement of endogenous antioxidants Materials and methods: Male Sprague-Dawley rats, divided into five groups (n = 7), were injected intraperitoneally once weekly for 2 weeks with 15 mg/kg AOM, except for the normal group (received saline). The animals were then administered orally for 8 weeks with 8% Tween-80 (vehicle; normal group), 8% Tween-80 (vehicle; cancer group) or, 50, 250 or 500 mg/kg MEMC. After treatments, colon samples were collected from each rat for the histopathological analysis, quantification of aberrant crypt foci formed and determination of colon antioxidant levels. MEMC was also subjected to HPLC analysis.
The extract exerted significant (p < 0.05): (i) anti-carcinogenesis activity, indicated by a decrease in the total aberrant crypt formation; (ii) antioxidant activity by increasing the colon tissue antioxidant markers [i.e., superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH)] and reducing the oxidant marker (i.e., malonaldehyde (MDA) levels in comparison with the cancer group. HPLC analysis demonstrated the presence of rutin.
Muntingia calabura leaves exert anticancer effect against AOM-induced colon cancer possibly via the action of flavonoids on the colon tissue antioxidant activity.
三叶赤楠（铁青树科）的叶子在传统医学实践中被广泛应用；科学研究发现其具有多种药理活性。然而，其抗癌作用尚未得到充分研究。
本研究旨在探讨 MEMC 对氧化偶氮甲烷（AOM）诱导的结肠癌的化学预防作用，并研究内源性抗氧化剂的参与情况。
雄性 Sprague-Dawley 大鼠分为五组（n=7），每周腹腔注射 15mg/kg AOM 一次，共 2 周，除正常组（给予生理盐水）外。然后，动物连续口服 8 周，给予 8%吐温-80（载体；正常组）、8%吐温-80（载体；癌症组）或 50、250 或 500mg/kg MEMC。治疗后，从每只大鼠收集结肠样本进行组织病理学分析、测定形成的异常隐窝焦点数量和测定结肠抗氧化水平。MEMC 也进行了 HPLC 分析。
提取物表现出显著的（p<0.05）：（i）抗癌活性，表现为总异常隐窝形成减少；（ii）抗氧化活性，通过增加结肠组织抗氧化标志物[即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽（GSH）]和降低氧化标志物（即丙二醛（MDA）水平）与癌症组相比。HPLC 分析表明存在芦丁。
三叶赤楠叶对 AOM 诱导的结肠癌具有抗癌作用，可能是通过类黄酮对结肠组织抗氧化活性的作用。
