Potter M R, Moore M
Immunology. 1979 May;37(1):187-94.
The spontaneous cytotoxicity of human peripheral blood lymphocyte preparations from normal donors for K562 target cells was examined. Effector cells were separated into SRBC rosette forming cell (RFC) and non-rosette forming cell (non-RFC) fractions using optimal and suboptimal rosetting procedures. RFC and non-RFC fractions both had high cytotoxic activity irrespective of the rosetting procedure. Owing to the larger size of the RFC fraction, it contained a higher proportion of the total activity in the preparation. Nylon fibre column adherent and non-adherent fractions also both produced cytotoxicity. Nylon fibre non-adherent cells separated by SRBC separation gave a RFC fraction with low activity and a non-RFC fraction with high activity. Separation of nylon fibre adherent cells gave RFC and non-RFC fractions with high cytotoxic activity. Therefore cytotoxic cells did not form a discrete subpopulation and either occur in several lymphocyte subsets or show a variable capacity to form SRBC rosettes and adhere to nylon fibre.
检测了来自正常供体的人外周血淋巴细胞制剂对K562靶细胞的自发细胞毒性。使用最佳和次优玫瑰花结形成程序将效应细胞分离为SRBC玫瑰花结形成细胞(RFC)和非玫瑰花结形成细胞(非RFC)组分。无论玫瑰花结形成程序如何,RFC和非RFC组分均具有高细胞毒性活性。由于RFC组分的尺寸较大,其在制剂中所含的总活性比例较高。尼龙纤维柱贴壁和非贴壁组分也均产生细胞毒性。通过SRBC分离法分离的尼龙纤维非贴壁细胞产生了低活性的RFC组分和高活性的非RFC组分。尼龙纤维贴壁细胞的分离产生了具有高细胞毒性活性的RFC和非RFC组分。因此,细胞毒性细胞并未形成一个离散的亚群,而是要么存在于几个淋巴细胞亚群中,要么表现出形成SRBC玫瑰花结和粘附于尼龙纤维的可变能力。