Ferriero D M, Sagar S M
Brain Res. 1987 Aug;431(2):207-14. doi: 10.1016/0165-3806(87)90209-4.
Levels of somatostatin-like immunoreactivity (SLI) were measured in the rat retina by radioimmunoassay during prenatal and postnatal development. SLI-containing cells were visualized by immunocytochemistry throughout this time period. SLI is present as early as embryonic day 15 (E15) and by E16 at concentrations 10-fold (4.4 +/- 1.4 pg/micrograms protein) higher than the adult retina, and the SLI is confined to cells in the inner neuroblastic layer. As the inner plexiform layer (IPL) develops (E17-18), the level of SLI falls (2.0 pg/micrograms protein) and distinct SLI neurites are seen throughout the inner neuroblastic layer. At birth (P0), the SLI level continues to decline at a time when cells are migrating. Few immunoreactive cells are seen in the developing ganglion cell layer (GCL), presumably as a result of this migration. At postnatal day 4 (P4), the amount of SLI continues to decline, and very few immunoreactive cells are noted. At P8, distinct cells contain SLI both in the GCL and inner nuclear layer (INL). SLI then increases as the retina matures postnatally. After eye opening, the amount of SLI approaches adult levels and the immunocytochemical pattern is similar to that reported for the adult rat retina; i.e. SLI is confined to two subpopulations of cells, one in the INL and one in the GCL. The early prenatal appearance of SLI suggests that it may play a role in retinal development.
在大鼠视网膜的产前和产后发育过程中，通过放射免疫分析法测量了生长抑素样免疫反应性（SLI）水平。在此期间，通过免疫细胞化学法观察到了含SLI的细胞。SLI早在胚胎第15天（E15）就已出现，到E16时，其浓度比成年视网膜高10倍（4.4±1.4 pg/微克蛋白质），且SLI局限于内神经母细胞层的细胞中。随着内网状层（IPL）的发育（E17 - 18），SLI水平下降（2.0 pg/微克蛋白质），并且在内神经母细胞层中可见明显的SLI神经突。出生时（P0），在细胞迁移时SLI水平持续下降。在发育中的神经节细胞层（GCL）中几乎看不到免疫反应性细胞，推测这是迁移的结果。在出生后第4天（P4），SLI的量继续下降，并且注意到免疫反应性细胞很少。在P8时，GCL和内核层（INL）中都有明显的含SLI细胞。然后，随着视网膜在出生后成熟，SLI增加。睁眼后，SLI的量接近成年水平，免疫细胞化学模式与成年大鼠视网膜的报道相似；即SLI局限于两个细胞亚群，一个在内核层，一个在神经节细胞层。SLI在产前早期出现表明它可能在视网膜发育中起作用。
