Fu Ganglan, Miao Liping, Wang Meng, Guo MingYan, Wang Chengli, Ji Fengtao, Cao Minghui
a Department of Anesthesiology , Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University , Guangzhou , China.
b Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation , Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University , Guangzhou , China.
Immunol Invest. 2017 Oct;46(7):647-662. doi: 10.1080/08820139.2017.1360337.
T helper cells play essential roles in anti-tumor immune response. However, the postoperative changes of peripheral T cell subsets and their clinical significance in breast cancer patients remain largely unknown.
We evaluated the perioperative changes of T lymphocyte subsets in invasive breast cancer (IBC) patients and breast fibroadenoma (BF) patients preoperatively (preop) and 6, 24, 72 hours postoperatively (POH6, POH24, and POH72). Proportions of CD3, CD4, CD8, T helper (Th) 1, Th2, Th17 cells, regulatory T cells (Treg), and CD4/CD8, Th1/Th2 ratio were detected by flow cytometry. Changes in T helper cell quantity were correlated to clinicopathological parameters. Furthermore, we explored the association between the perioperative variations of T cell subsets and disease-free survival (DFS) of IBC patients.
In IBC patients, Th1 cells diminished while Tregs elevated in postoperative 72 hours in the peripheral blood. In contrast, no significant perioperative changes of T cell subsets were observed in BF patients. Postoperative lower Th1 cells at POH 72 of IBC patients were correlated with greater tumor burden, HER2 positive and Ki67 positive. The increased Tregs at POH 72 of IBC patients were correlated with larger tumor size and HER2 positive. Th1 cell decline and Treg increment were both associated with shorter DFS in IBC patients.
The variations of peripheral T helper cell subsets showed postoperative immunosuppression and were associated with poor prognosis in IBC patients.
辅助性T细胞在抗肿瘤免疫反应中发挥着重要作用。然而，乳腺癌患者外周血T细胞亚群的术后变化及其临床意义仍 largely unknown。
我们评估了浸润性乳腺癌（IBC）患者和乳腺纤维瘤（BF）患者术前（preop）以及术后6、24、72小时（POH6、POH24和POH72）外周血T淋巴细胞亚群的围手术期变化。通过流式细胞术检测CD3、CD4、CD8、辅助性T细胞（Th）1、Th2、Th17细胞、调节性T细胞（Treg）的比例以及CD4/CD8、Th1/Th2比值。辅助性T细胞数量的变化与临床病理参数相关。此外，我们探讨了IBC患者T细胞亚群围手术期变化与无病生存期（DFS）之间的关联。
在IBC患者中，外周血Th1细胞在术后72小时减少而Tregs升高。相比之下，BF患者未观察到T细胞亚群有明显的围手术期变化。IBC患者术后72小时较低的Th1细胞与更大的肿瘤负荷、HER2阳性和Ki67阳性相关。IBC患者术后72小时Tregs增加与更大的肿瘤大小和HER2阳性相关。Th1细胞减少和Treg增加均与IBC患者较短的DFS相关。
外周辅助性T细胞亚群的变化显示出术后免疫抑制，并与IBC患者的不良预后相关。
