日本石龟（Amyda japonica）血管紧张素I转换酶活性的特征分析

Characterization of angiotensin I-converting enzyme activity in the freshwater turtle, Amyda japonica.

Cho K W, Kim S Z, Kim S H, Koh G Y, Seul K H

Comp Biochem Physiol A Comp Physiol. 1987;87(3):645-8. doi: 10.1016/0300-9629(87)90376-8.

Abstract

Angiotensin I-Converting Enzyme (ACE) activity has been characterized in the freshwater turtle, Amyda japonica. 2. Peak activity of ACE in plasma from the freshwater turtle was shown at pH 9.0, which was more alkaline compared to that of mammals. 3. Chloride requirements for the optimal ACE activity were different from species. 4. ACE inhibitors, EDTA, teprotide (SQ 20,881), Captopril (SQ 14,225) showed dose-dependent inhibitions of ACE activity in plasma from the freshwater turtle as well as mammals. 5. ACE activity was found in several different tissues with a different activity showing the highest activity in kidney homogenate from the freshwater turtle, Amyda japonica.

摘要

已对淡水龟日本石龟的血管紧张素I转换酶（ACE）活性进行了表征。2. 淡水龟血浆中ACE的峰值活性在pH 9.0时出现，与哺乳动物相比，该pH值更偏碱性。3. 不同物种对ACE最佳活性的氯离子需求不同。4. ACE抑制剂乙二胺四乙酸（EDTA）、替普罗肽（SQ 20,881）、卡托普利（SQ 14,225）对淡水龟和哺乳动物血浆中的ACE活性均表现出剂量依赖性抑制作用。5. 在几种不同组织中发现了ACE活性，不同组织的活性不同，日本石龟肾脏匀浆中的活性最高。