Peng Li-Qing, Yu Wen-Yan, Xu Jing-Jing, Cao Jun
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
The Emergency Department, Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310018, China.
Food Chem. 2018 Jan 15;239:1075-1084. doi: 10.1016/j.foodchem.2017.07.031. Epub 2017 Jul 10.
A simple, green and effective extraction method, namely, pyridinium ionic liquid- (IL) based liquid-solid extraction (LSE), was first designed to extract the main inorganic and organic iodine compounds (I, monoiodo-tyrosine (MIT) and diiodo-tyrosine (DIT)). The optimal extraction conditions were as follows: ultrasonic intensity 100W, IL ([EPy]Br) concentration 200mM, extraction time 30min, liquid/solid ratio 10mL/g, and pH value 6.5. The morphologies of Laminaria were studied by scanning electron microscopy and transmission electron microscopy. The recovery values of I, MIT and DIT from Laminaria were in the range of 88% to 94%, and limits of detection were in the range of 59.40 to 283.6ng/g. The proposed method was applied to the extraction and determination of iodine compounds in three Laminaria. The results showed that IL-based LSE could be a promising method for rapid extraction of bioactive iodine from complex food matrices.
首次设计了一种简单、绿色且有效的萃取方法，即基于吡啶鎓离子液体（IL）的液固萃取（LSE），用于萃取主要的无机和有机碘化合物（I、一碘酪氨酸（MIT）和二碘酪氨酸（DIT））。最佳萃取条件如下：超声强度100W、IL（[EPy]Br）浓度200mM、萃取时间30分钟、液固比10mL/g以及pH值6.5。通过扫描电子显微镜和透射电子显微镜研究了海带的形态。海带中I、MIT和DIT的回收率在88%至94%范围内，检测限在59.40至283.6ng/g范围内。所提出的方法应用于三种海带中碘化合物的萃取和测定。结果表明，基于IL的LSE可能是一种从复杂食品基质中快速萃取生物活性碘的有前景的方法。
