糖醛酸组成对来自突尼斯三种不同属褐藻的海藻酸盐胃保护活性的影响。

Influence of the uronic acid composition on the gastroprotective activity of alginates from three different genus of Tunisian brown algae.

作者信息

Ammar Hiba Hadj, Lajili Sirine, Sakly Nawfel, Cherif Dora, Rihouey Christophe, Le Cerf Didier, Bouraoui Abderrahman, Majdoub Hatem

机构信息

Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019 Monastir, Tunisia.

Laboratoire de développement chimique, galénique et pharmacologique des médicaments, Faculté de Pharmacie de Monastir, Université de Monastir, 5000 Monastir, Tunisia.

出版信息

Food Chem. 2018 Jan 15;239:165-171. doi: 10.1016/j.foodchem.2017.06.108. Epub 2017 Jun 21.

DOI:10.1016/j.foodchem.2017.06.108

PMID:28873554

Abstract

Alginates from three genus of Tunisian brown algae were isolated and characterized by size exclusion chromatography and Solid-state NMR spectroscopy. Alginate from Padina pavonica (APP) had the highest molecular weight (Mw) with 147,000g/mol while it was 85,000g/mol for alginate from Cystoseira compressa (ACC) and 58,000g/mol for alginate from Dictyopteris membranaceae (ADM). The mannuronate (M) to guluronate (G) ratios were estimated from spectral deconvolution of the C CP/MAS spectra and the results has shown that all the extracts are mannuronic acid-rich alginates with M/G ratio increased in the order ADM - ACC - APP. An interesting gastroprotective effect was observed for the extracts; ADM and ACC exhibited the highest inhibition of gastric lesions, at 50mg/kg, with 83.41% and 75.39% respectively. Otherwise, it has been shown that the gastroprotective effect of alginates depends mainly on their uronic acid composition.

摘要

从突尼斯三种褐藻中分离出藻酸盐，并通过尺寸排阻色谱法和固态核磁共振光谱对其进行表征。来自波氏仙掌藻（APP）的藻酸盐具有最高的分子量（Mw），为147,000g/mol，而来自密挤囊链藻（ACC）的藻酸盐分子量为85,000g/mol，来自膜质网地藻（ADM）的藻酸盐分子量为58,000g/mol。通过对碳交叉极化/魔角旋转（C CP/MAS）光谱进行谱图反褶积来估算甘露糖醛酸（M）与古洛糖醛酸（G）的比例，结果表明所有提取物均为富含甘露糖醛酸的藻酸盐，其M/G比例按ADM - ACC - APP的顺序增加。提取物表现出有趣的胃保护作用；ADM和ACC在50mg/kg时对胃部损伤的抑制作用最强，分别为83.41%和75.39%。此外，研究表明藻酸盐的胃保护作用主要取决于其糖醛酸组成。