Machado Kamilla Nunes, Freitas Aline Alves de, Cunha Luzia Helena, Faraco André Augusto Gomes, Pádua Rodrigo Maia de, Braga Fernão Castro, Vianna-Soares Cristina Duarte, Castilho Rachel Oliveira
Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais (UFMG), Av. Pres. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil.
Fundação Ezequiel Dias (FUNED), Rua Conde Pereira Carneiro 80, Belo Horizonte, Minas Gerais, Brazil.
Food Chem. 2018 Jan 15;239:180-188. doi: 10.1016/j.foodchem.2017.06.089. Epub 2017 Jun 16.
Paullinia cupana is a plant native to Brazil that is widely used in traditional medicine as a physical and mental stimulant. It is also used worldwide to produce soft drinks. A method for the simultaneous quantitation of seven markers in guaraná by HPLC-PDA was developed, and extraction methods for the determination of methylxanthines and tannins were investigated. Quantified substances were theobromine, theophylline, caffeine, catechin, epicatechin, procyanidins A2 and B2. Results confirmed the satisfactory selectivity and linearity (r≥0.99) within the mass ranges. Repeatability (RSD≤2.80%), intermediate precision (RSD≤4.47%), accuracy (recoveries from 90.59%-104.67%), and robustness were demonstrated. Extract 1 presented the contents: 0.0177% (±1.02%) for theobromine, 0.0131% (±1.14%) for theophylline, 2.9429% (±1.27%) for caffeine, 0.4563% (±1.02%) for catechin, 0.5515% (±1.05%) for epicatechin, 0.0607% (±2.80%) for A2 and 0.1035% (±1.39%) for B2. The method for simultaneous quantitation of seven chemical markers in guaraná proved to be reliable using a simple and convenient HPLC setup.
瓜拉那是一种原产于巴西的植物，在传统医学中被广泛用作身心兴奋剂。它在全球范围内还被用于生产软饮料。开发了一种通过高效液相色谱-光电二极管阵列检测法（HPLC-PDA）同时定量瓜拉那中七种标志物的方法，并研究了用于测定甲基黄嘌呤和单宁的提取方法。定量的物质有可可碱、茶碱、咖啡因、儿茶素、表儿茶素、原花青素A2和B2。结果证实了在质量范围内具有令人满意的选择性和线性（r≥0.99）。展示了方法的重复性（RSD≤2.80%）、中间精密度（RSD≤4.47%）、准确度（回收率为90.59%-104.67%）以及稳健性。提取物1的含量为：可可碱0.0177%（±1.02%）、茶碱0.0131%（±1.14%）、咖啡因2.9429%（±1.27%）、儿茶素0.4563%（±1.02%）、表儿茶素0.5515%（±1.05%）、A2为0.0607%（±2.80%）、B2为0.1035%（±1.39%）。使用简单便捷的HPLC装置，同时定量瓜拉那中七种化学标志物的方法被证明是可靠的。
