Jastrzębska Aneta, Piasta Anna, Krzemiński Marek, Szłyk Edward
Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarin 7 Str., 87-100 Toruń, Poland.
Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarin 7 Str., 87-100 Toruń, Poland.
Food Chem. 2018 Jan 15;239:225-233. doi: 10.1016/j.foodchem.2017.06.100. Epub 2017 Jun 20.
3,5-Bis-(trifluoromethyl)phenyl isothiocyanate, was used as a convenient reagent for the derivatization of histamine, tyramine, tryptamine and 2-phenylethylamine, which eliminates the purification step. The obtained derivatives were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Additionally, the total amount of these amines was determined by F nuclear magnetic resonance analysis. The procedures were optimized and validated for linearity, limit of detection and quantification, intra- and inter-day precision and recovery resulting in good reproducibility and accuracy. The reagent was applied for determination of the above mentioned biogenic amines in beverages, and permitted an undemanding separation and determination of the derivatives. Moreover, it is a convenient reagent for analysis of the total amine content by quantitative F NMR.
3,5-双（三氟甲基）苯基异硫氰酸酯被用作组胺、酪胺、色胺和2-苯乙胺衍生化的便捷试剂，省去了纯化步骤。所得衍生物通过液相色谱-串联质谱法（LC-MS/MS）进行测定。此外，这些胺类的总量通过氟核磁共振分析来测定。该方法针对线性、检测限和定量限、日内和日间精密度以及回收率进行了优化和验证，具有良好的重现性和准确性。该试剂用于测定饮料中上述生物胺，能够轻松实现衍生物的分离和测定。此外，它还是通过定量氟核磁共振分析总胺含量的便捷试剂。
