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采用反相高效液相色谱-四极杆飞行时间质谱联用技术鉴定不同发酵剂和成熟时间干发酵骆驼香肠中的抗氧化肽和降压肽。

Identification of peptides with antioxidant and antihypertensive capacities by RP-HPLC-Q-TOF-MS in dry fermented camel sausages inoculated with different starter cultures and ripening times.

机构信息

Departmento de Química Analítica, Química Física e Ingeniería Química, Facultad de Biología, Ciencias Ambientales y Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.; Research Unity Bio-Preservation and Valorisation of Agricultural Product, High Food Industries School of Tunisia, 58 Alain Savary, Tunis El Khadra 1003, Tunisia.

Departmento de Química Analítica, Química Física e Ingeniería Química, Facultad de Biología, Ciencias Ambientales y Química, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares (Madrid), Spain.

出版信息

Food Res Int. 2017 Oct;100(Pt 1):708-716. doi: 10.1016/j.foodres.2017.07.072. Epub 2017 Aug 2.

Abstract

Low molecular weight peptides are produced during meat fermentation. They contribute to generate flavor compounds but they can also exert certain bioactivities. The aim of this work was to evaluate, for the first time, the generation of bioactive peptides during the preparation of dry fermented camel sausages and to study the influence of the ripening time and the starter culture on bacteria growing, peptide concentration and size, and antioxidant and antihypertensive capacities of peptides. Camel meat sausages inoculated with different starter bacteria and non-inoculated were ripened up to 28days. Results demonstrated that bacteria population, peptide concentration, and peptide size were affected by the ripening time and the inoculated bacteria. Moreover, the ripening process resulted in an increasing antioxidant and antihypertensive capacity showing the highest bioactivities in fractions with peptides below 3kDa. Peptides in these fractions were identified by RP-HPLC-Q-TOF-MS analysis. Identified peptides showed common features with peptides with antioxidant or antihypertensive activity.

摘要

在肉类发酵过程中会产生低分子量肽。它们有助于生成风味化合物,但也可以发挥某些生物活性。本工作的目的是首次评估干发酵骆驼香肠制备过程中生物活性肽的生成,并研究成熟时间和发酵剂对细菌生长、肽浓度和大小以及肽的抗氧化和降压能力的影响。接种不同发酵剂和未接种的骆驼肉香肠成熟至 28 天。结果表明,细菌种群、肽浓度和肽大小受成熟时间和接种细菌的影响。此外,成熟过程会增加抗氧化和降压能力,在 3kDa 以下的肽段中显示出最高的生物活性。通过 RP-HPLC-Q-TOF-MS 分析鉴定了这些肽段中的肽。鉴定出的肽具有与具有抗氧化或降压活性的肽的共同特征。

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