在狒狒卵母细胞培养中用三种不同血清补充哈姆氏F10培养基。
Supplementation of Ham's F10 culture medium with three different sera in the culturing of baboon oocytes.
作者信息
Fourie F R, Snyman E, van der Merwe J V
出版信息
Comp Biochem Physiol A Comp Physiol. 1987;87(4):1103-6. doi: 10.1016/0300-9629(87)90046-6.
PMID:2887377
Abstract
- Ten female baboons (Papio ursinus) were stimulated for a total of 20 cycles with 3 ovulation induction agents. 2. Oocytes obtained were randomly allocated to Ham's F10 culture medium supplemented with human fetal cord serum, primate serum or commercial fetal bovine serum respectively. 3. Fertilization occurred (38.1-45.5%) in all 3 supplements, but cleavage and embryo development was more successful in culture medium supplemented with fetal bovine serum. 4. Eight embryos were cultured and 6 (75%) of these were cultured in fetal bovine serum supplemented medium.
摘要
- 用3种促排卵剂对10只雌性狒狒（山魈）进行了总共20个周期的刺激。2. 获得的卵母细胞分别随机分配到添加人脐带血清、灵长类动物血清或商业胎牛血清的哈姆F10培养基中。3. 在所有3种补充剂中均发生了受精（38.1%-45.5%），但在添加胎牛血清的培养基中，卵裂和胚胎发育更为成功。4. 培养了8个胚胎，其中6个（75%）在添加胎牛血清的培养基中培养。
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