a Department of Food Science and Biotechnology , Graduate School, Kyungpook National University , Daegu , Korea.
b Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute , Korea Atomic Energy Research Institute , Jeongeup , Korea.
Pharm Biol. 2017 Dec;55(1):2110-2115. doi: 10.1080/13880209.2017.1371770.
Aloe has been used for the prevention and cure of various diseases and symptoms including burns, injuries, oedema and pain.
This study determines the specific inhibitory activity of matrix metalloproteinase (MMP)-9 induced by the low molecular-weight gel fraction of Aloe vera (L.) Burm.f. (lgfAv) on alcohol-induced acute gastric lesions.
We examined the protective effects of oral (p.o.) administration of lgfAv (molecular weight cutoff <50.0 kDa, 150.0 mg/kg body weight) in a Balb/c mouse model of alcohol-induced acute gastritis for 1 h exposure. By measuring ulcer index, we compared the antiulcerative activity of the fraction. mRNA expression and immunohistochemical analysis of various biomarkers were performed.
The lgfAv-treated mice exhibited drastically fewer ulcer lesions than the untreated control mice did. It featured that lgfAv lessened the ulcer lesions than their relevant controls. Moreover, the transcriptional level of MMP-9 was completely alleviated by lgfAv treatment in alcohol-treated gastritis-induced mice.
The transcriptional level of MMP-9 was significantly alleviated by lgfAv treatment of the model. However, reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry experiments revealed that lgfAv treatment in mucosal tissues had the potential to inhibit the mRNA and protein expression levels of MMP-9, respectively. The protein expression of MMP-9 was closely associated with lgfAv-induced gastroprotection against alcohol-induced gastric lesions.
The present findings suggest that lgfAv has the potential to alleviate alcohol-induced acute gastric lesions, which is mediated in part, mainly by the suppression of the mRNA expression of MMP-9.
芦荟已被用于预防和治疗各种疾病和症状，包括烧伤、损伤、水肿和疼痛。
本研究旨在确定低分子质量凝胶部分的芦荟（Aloe vera（L.）Burm.f.）（lgfAv）对酒精诱导的急性胃损伤中基质金属蛋白酶（MMP）-9的特定抑制活性。
我们在Balb/c 小鼠酒精诱导的急性胃炎模型中，通过口服（p.o.）给予 lgfAv（分子量截止值<50.0 kDa，150.0 mg/kg 体重），1 h 后观察其对胃的保护作用。通过测量溃疡指数，比较该部分的抗溃疡活性。进行了各种生物标志物的 mRNA 表达和免疫组织化学分析。
与未处理的对照组相比，lgfAv 处理的小鼠溃疡病变明显减少。lgfAv 减轻了溃疡病变。此外，lgfAv 治疗可完全减轻酒精性胃炎诱导的小鼠 MMP-9 的转录水平。
lgfAv 治疗显著减轻 MMP-9 的转录水平。然而，逆转录聚合酶链反应（RT-PCR）和免疫组织化学实验表明，lgfAv 处理在粘膜组织中分别具有抑制 MMP-9 的 mRNA 和蛋白表达水平的潜力。MMP-9 的蛋白表达与 lgfAv 诱导的抗酒精诱导的胃损伤密切相关。
本研究结果表明，lgfAv 具有减轻酒精诱导的急性胃损伤的潜力，部分机制可能是通过抑制 MMP-9 的 mRNA 表达。
